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VPS25 Polyclonal Antibody

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Antibody Testing Data (10)

VPS25 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

VPS25 Antibody (15669-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 15669-1-AP) (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

15669-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

VPS25 Fusion Protein Ag8134 (1-176 aa encoded by BC006282)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAMSFEWPW QYRFPPFFTL QPNVDTRQKQ LAAWCSLVLS FCRLHKQSSM TVMEAQESPL FNNVKLQRKL PVESIQIVLE ELRKKGNLEW LDKSKSSFLI MWRRPEEWGK LIYQWVSRSG QNNSVFTLYE LTNGEDTEDE EFHGLDEATL LRALQALQQE HKAEIITVSD GRGVKFF (1-176 aa encoded by BC006282)

Target Information

VPS25 (Vacuolar Protein Sorting 25 Homolog) is a Protein Coding gene. Among its related pathways are Delta508-CFTR traffic / ER-to-Golgi in CF and Budding and maturation of HIV virion. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and protein N-terminus binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 EST 2022

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

PLoS pathogens

Liu CC,Liu YY,Zhou JF,Chen X,Chen H,Hu JH,Chen J,Zhang J,Sun RC,Wei JC,Go YY,Morita E,Zhou B

Tue Feb 01 00:00:00 EST 2022

Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.

Frontiers in microbiology

Ruan Z,Liang Y,Chen Z,Yin J,Li C,Pan P,Zhang Q,Wu J,Luo Z

Tue Oct 25 00:00:00 EDT 2022

Enterovirus 71 non-structural protein 3A hijacks vacuolar protein sorting 25 to boost exosome biogenesis to facilitate viral replication.

Frontiers in microbiology

Ruan Z,Liang Y,Chen Z,Yin J,Li C,Pan P,Zhang Q,Wu J,Luo Z

Tue Oct 25 00:00:00 EDT 2022

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Cancer cell international

Zhu X,Yang H,Zhang M,Wu X,Jiang L,Liu X,Lv K

Sat Dec 04 00:00:00 EST 2021

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Cancer cell international

Zhu X,Yang H,Zhang M,Wu X,Jiang L,Liu X,Lv K

Sat Dec 04 00:00:00 EST 2021

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Cancer cell international

Zhu X,Yang H,Zhang M,Wu X,Jiang L,Liu X,Lv K

Sat Dec 04 00:00:00 EST 2021

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Cancer cell international

Zhu X,Yang H,Zhang M,Wu X,Jiang L,Liu X,Lv K

Sat Dec 04 00:00:00 EST 2021

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 EDT 2020

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Current biology : CB

Karasmanis EP,Hwang D,Nakos K,Bowen JR,Angelis D,Spiliotis ET

Mon Jul 08 00:00:00 EDT 2019

VPS37A directs ESCRT recruitment for phagophore closure.

The Journal of cell biology

Takahashi Y,Liang X,Hattori T,Tang Z,He H,Chen H,Liu X,Abraham T,Imamura-Kawasawa Y,Buchkovich NJ,Young MM,Wang HG

Mon Oct 07 00:00:00 EDT 2019

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Nature communications

Scheffer LL,Sreetama SC,Sharma N,Medikayala S,Brown KJ,Defour A,Jaiswal JK

Tue Dec 23 00:00:00 EST 2014

Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.

Nature communications

Scheffer LL,Sreetama SC,Sharma N,Medikayala S,Brown KJ,Defour A,Jaiswal JK

Tue Dec 23 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25; HGNC:28122; hVps25; MGC10540; Vacuolar protein-sorting-associated protein 25

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Gene Aliases: 1110020N13Rik; AW107467; D11Wsu68e; DERP9; EAP20; FAP20; VPS25

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UniProt ID: (Human) Q9BRG1, (Rat) P0C0A1, (Mouse) Q9CQ80

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Entrez Gene ID: (Human) 84313, (Rat) 681059, (Mouse) 28084

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Function(s)

structural molecule activity protein binding protein homodimerization activity protein N-terminus binding molecular_function protein kinase activity ATP binding membrane traffic protein

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated autophagy endosomal transport multivesicular body assembly protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway regulation of viral life cycle protein phosphorylation biological_process protein transport transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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VPS25 Polyclonal Antibody

VPS25 Antibody (15669-1-AP) in IHC (P)

Product Details

15669-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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