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Application
FIGURE: 1 / 6
VPS33B Antibody (PA5-100052) in ICC/IF
Immunofluorescent analysis of VPS33B in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and VPS33B polyclonal antibody (Product # PA5-100052) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human VPS33B(Accession Q9H267), corresponding to amino acid residues A454-K504.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815582
Product Specific Information
Antibody detects endogenous levels of total VPS33B.
Target Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ14848; hVPS33B; r-vps33b; vacuolar protein sorting 33 homolog B; vacuolar protein sorting 33-like protein B; vacuolar protein sorting 33B; vacuolar protein sorting homolog r-vps33b; Vacuolar protein sorting-associated protein 33B; vacuolar sorting protein 33b; vps33b
Gene Aliases: VPS33B; zgc:110803
UniProt ID: (Human) Q9H267, (Mouse) P59016, (Rat) Q63616, (Zebrafish) Q58EN8
Entrez Gene ID: (Human) 26276, (Mouse) 233405, (Rat) 64060, (Zebrafish) 541534
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