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VPS34 Antibody (MA5-50485) in ICC/IF
Immunocytochemical analysis of VPS34 using VPS34 Recombinant Monoclonal Antibody (Product # MA5-50485) in HeLa cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS34 stained by VPS34 antibody (MA5-50485) diluted at 1:500. Blue: Fluoroshield with DAPI.
Product Details
MA5-50485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL2305
Immunogen
Recombinant fragmemt of human VPS34.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094210
Target Information
PIK3C3 (hVPS34) is the sole class III member of the PI3 Kinase family. PIK3C3 phosphorylates phosphatidylinositol (PI) to produce phosphatidylinositol 3-phosphate [PI(3)P], which recruits downstream effectors containing FYVE and Phox domains to membranes. PIK3C3 is involved in coupling nutrient signaling to mTOR and also plays a role in autophagy and intracellular trafficking. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: catalytic phosphatidylinositol 3-kinase 3; catalytic subunit type 3 Antibody; Class III PI 3-kinase; hVps34; hVps34 Antibody; OTTMUSP00000023341; OTTMUSP00000023350; phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; phosphatidylinositol 3-kinase, catalytic subunit type 3; Phosphoinositide-3-kinase class 3; phosphoinositide-3-kinase, class 3; PI-3K Vps34p; PI3-kinase type 3; PI3K type 3; pi3kc3; PIK3C3 Antibody; PtdIns-3-kinase type 3
Gene Aliases: 5330434F23Rik; hVps34; PIK3C3; VPS34
UniProt ID: (Human) Q8NEB9, (Rat) O88763, (Mouse) Q6PF93
Entrez Gene ID: (Dog) 490477, (Human) 5289, (Rat) 65052, (Mouse) 225326
protein kinase activity
protein binding
ATP binding
1-phosphatidylinositol-3-kinase activity
phosphatidylinositol 3-kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
kinase
autophagosome assembly
cytokinesis
protein phosphorylation
phosphatidylinositol biosynthetic process
endosome organization
macroautophagy
protein processing
regulation of cytokinesis
toll-like receptor 9 signaling pathway
phosphatidylinositol-3-phosphate biosynthetic process
cellular response to glucose starvation
response to leucine
early endosome to late endosome transport
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of protein secretion
endocytosis
autophagy
cell cycle
endosomal transport
phosphorylation
pexophagy
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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