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Product Details
PA5-95636
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human WEE1 (ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807438
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human placenta tissue, human A431 whole cell, human U2OS whole cell. IHC: human mammary cancer tissue. ICC/IF: U20S cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686I18166; FLJ16446; wee 1 homolog; WEE1 homolog; WEE1 tyrosine kinase; WEE1+ homolog; Wee1-like protein kinase; Wee1A kinase; WEE1hu
Gene Aliases: WEE1; WEE1A; WEE1hu
UniProt ID: (Human) P30291, (Mouse) P47810, (Rat) Q63802
Entrez Gene ID: (Human) 7465, (Mouse) 22390, (Rat) 308937
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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