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Application
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WISP1 Antibody (PA5-80228) in IHC (P)
Immunohistochemistry (Paraffin) analysis of WISP1 in paraffin-embedded section of human testicular germ cell tumor tissue using WISP1 Polyclonal Antibody (Product # PA5-80228). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 2 µg/mL dilut... View More
Product Details
PA5-80228
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1 (354-367aa ADLESYPDFSEIAN).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747342
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, mouse kidney tissue. IHC: human colorectal adenocarcinoma tissue, human testicular germ cell tumor tissue.
Target Information
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCN family member 4; CTC-458A3.8; ELM-1; WISP-1; Wnt-1 inducible signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1 induced secreted protein 1; WNT1-inducible-signaling pathway protein 1
Gene Aliases: AW146261; CCN4; Elm1; WISP1; WISP1c; WISP1i; WISP1tc
UniProt ID: (Human) O95388, (Rat) Q99PP0, (Mouse) O54775
Entrez Gene ID: (Human) 8840, (Rat) 65154, (Mouse) 22402
Performance Guarantee
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