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Invitrogen

WRN Polyclonal Antibody

1 Reference

View all (22) WRN antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)

Application

WRN Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

WRN Antibody (PA5-27319) in ICC/IF

Immunocytochemistry (Confocal immunofluorescence) of WRN in paraformaldehyde-fixed HeLa. Samples were incubated with WRN polyclonal antibody (Product # PA5-27319) using a dilution of 1:500 (green). Alpha-tubulin filaments were labeled with alpha Tubulin monoclonal antibody (Product # MA1-25054) (Red) at 1:2,500.

WRN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA5-27319

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human WRN. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544795

Product Specific Information

Recommended positive controls: 293T, A431.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.

Nature cell biology

Ponsioen B,Post JB,Buissant des Amorie JR,Laskaris D,van Ineveld RL,Kersten S,Bertotti A,Sassi F,Sipieter F,Cappe B,Mertens S,Verlaan-Klink I,Boj SF,Vries RGJ,Rehmann H,Vandenabeele P,Riquet FB,Trusolino L,Bos JL,Snippert HJG

PA5-27319 was used in Western Blot to provide a mechanistic understanding of the effectivity of EGFR inhibitors within combination therapies against KRAS and BRAF mutant CRC.

Thu Apr 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DKFZp686C2056; DNA helicase, RecQ-like type 3; exonuclease WRN; RecQ protein-like 2; Werner syndrome protein; Werner syndrome, RecQ helicase-like

Gene Aliases: RECQ3; RECQL2; RECQL3; WRN

UniProt ID: (Human) Q14191

Entrez Gene ID: (Human) 7486

Function(s)

magnesium ion binding Y-form DNA binding bubble DNA binding DNA binding DNA helicase activity chromatin binding ATP-dependent DNA helicase activity helicase activity exonuclease activity protein binding ATP binding 3'-5' exonuclease activity four-way junction helicase activity ATPase activity manganese ion binding protein complex binding protein homodimerization activity 3'-5' DNA helicase activity ATP-dependent 3'-5' DNA helicase activity G-quadruplex DNA binding DNA helicase

Process(es)

telomere maintenance via recombination telomere maintenance DNA synthesis involved in DNA repair strand displacement replicative cell aging DNA metabolic process DNA replication base-excision repair double-strand break repair cellular response to DNA damage stimulus response to oxidative stress brain development aging cell aging cellular response to starvation response to UV-C multicellular organism aging protein sumoylation replication fork processing nucleolus to nucleoplasm transport DNA duplex unwinding regulation of growth rate regulation of apoptotic process positive regulation of hydrolase activity cellular response to gamma radiation nucleic acid phosphodiester bond hydrolysis regulation of signal transduction by p53 class mediator

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