Invitrogen

XBP1 Recombinant Superclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (26) XBP1 antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

711490

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

Protein corresponding to human XBP1 (aa1-aa185)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2716901

Product Specific Information

This antibody is predicted to react with Monkey, Pig, Bovine, Mouse

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000028682; Tax-responsive element-binding protein 5; TREB-5; X-box-binding protein 1; XBP-1

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Gene Aliases: TREB-5; TREB5; XBP-1; XBP1; XBP2

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UniProt ID: (Human) P17861

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Entrez Gene ID: (Human) 7494

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity
Process(es)
angiogenesis liver development endothelial cell proliferation positive regulation of immunoglobulin production transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process fatty acid biosynthetic process autophagy immune response positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response muscle organ development regulation of autophagy negative regulation of myotube differentiation protein transport endoplasmic reticulum unfolded protein response exocrine pancreas development protein destabilization cellular response to nutrient cellular response to insulin stimulus cellular triglyceride homeostasis IRE1-mediated unfolded protein response ATF6-mediated unfolded protein response cellular response to glucose starvation cholesterol homeostasis negative regulation of apoptotic process positive regulation of MHC class II biosynthetic process positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter neuron development positive regulation of immunoglobulin secretion fatty acid homeostasis sterol homeostasis adipose tissue development epithelial cell maturation involved in salivary gland development intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to fructose stimulus cellular response to glucose stimulus cellular response to interleukin-4 cellular response to peptide hormone stimulus positive regulation of plasma cell differentiation positive regulation of endoplasmic reticulum unfolded protein response positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of lactation response to insulin-like growth factor stimulus positive regulation of hepatocyte proliferation positive regulation of interleukin-6 secretion
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