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Invitrogen

YBX1 Recombinant Rabbit Monoclonal Antibody (9J7M4)

View all (34) YBX1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)

Application

YBX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

YBX1 Antibody (MA5-42819) in ICC/IF

Immunofluorescence analysis of YBX1 in NIH-3T3 cells. Samples were incubated with YBX1 Monoclonal antibody (Product # MA5-42819) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

YBX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-42819

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

Immunocytochemistry (ICC/IF)

1:200-1:800

ELISA (ELISA)

1 µg/mL

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9J7M4

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 225-324 of human YB-1/YBX1 (P67809).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2911960

Product Specific Information

Positive test controls include: Mouse testis, Rat heart, HeLa. The target is usually found in the following locations: Cytoplasm, Cytoplasmic granule, Nucleus, Secreted.

Immunogen sequence: GAGEQGRPVR QNMYRGYRPR FRRGPPRQRQ PREDGNEEDK ENQGDETQGQ QPPQRRYRRN FNYRRRRPEN PKPQDGKETK AADPPAENSS APEAEQGGAE

Target Information

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y box transcription factor; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1

Gene Aliases: 1700102N10Rik; BP-8; Byb1; C79409; CBF-A; Cbfa; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1

UniProt ID: (Human) P67809, (Mouse) P62960, (Rat) P62961

Entrez Gene ID: (Human) 4904, (Mouse) 22608, (Rat) 500538

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding double-stranded DNA binding single-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA binding protein binding poly(A) RNA binding GTPase binding p53 binding nucleic acid binding chromatin binding mRNA binding transcription factor binding sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mRNA processing positive regulation of cell proliferation RNA splicing negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway negative regulation of striated muscle cell differentiation positive regulation of cell division CRD-mediated mRNA stabilization mRNA splicing, via spliceosome protein localization to cytoplasmic stress granule

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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