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YBX1 Antibody (MA5-41129) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of YBX1 in HepG2 cells (green) using YBX1 Recombinant Monoclonal Antibody (Product # MA5-41129). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41129
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ST0432
Immunogen
recombinant protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2898883
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BP8; CBF-A; CCAAT-binding; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; DBPB; DNA binding protein B; DNA-binding protein B; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MGC104858; MGC110976; MGC117250; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y box transcription factor; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1
Gene Aliases: 1700102N10Rik; BP-8; Byb1; C79409; CBF-A; Cbfa; CSDA2; CSDB; DBPB; EF1A; EFI-A; MDR-NF1; Msy-1; MSY1; mYB-1a; NSEP-1; NSEP1; YB-1; YB1; YBX1
UniProt ID: (Human) P67809, (Mouse) P62960, (Rat) P62961
Entrez Gene ID: (Human) 4904, (Mouse) 22608, (Rat) 500538
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
poly(A) RNA binding
GTPase binding
p53 binding
nucleic acid binding
chromatin binding
mRNA binding
transcription factor binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mRNA processing
positive regulation of cell proliferation
RNA splicing
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
negative regulation of striated muscle cell differentiation
positive regulation of cell division
CRD-mediated mRNA stabilization
mRNA splicing, via spliceosome
protein localization to cytoplasmic stress granule
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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