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Proteintech

ZBTB32 Polyclonal Antibody

View all (9) TZFP antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Application

ZBTB32 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

ZBTB32 Antibody (12516-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG (H+L), 21738-1-AP 00069062 (red).

ZBTB32 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

12516-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:800

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ZBTB32 fusion protein Ag3199

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: MSLPPIRLPS PYGSDRLVQL AARLRPALCD TLITVGSQEF PAHSLVLAGV SQQLGRRGQW ALGEGISPST FAQLLNFVYG ESVELQPGEL RPLQEAARAL GVQSLEEACW RARGDRAKKP DPGLKKHQEE PEKPSRNPER ELGDPGEKQK PEQVSRTGGR EQEMLHKHSP PRGRPEMAGA TQEAQQEQTR SKEKRLQAPV GQRGADGKHG VLTWLRENPG GSEESLRKLP GPLPPAGSLQ TSVTPRPSWA EAPWLVGGQP ALWSILLMPP RYGIPFYHST PTTGAWQEVW REQRRTCNLC GS

Target Information

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FANCC-interacting protein; Fanconi anemia zinc finger protein; GATA repressor; OTTHUMP00000045790; Repressor of GATA; Testis zinc finger protein; Zinc finger and BTB domain-containing protein 32; Zinc finger protein 538

Gene Aliases: 4930524C15Rik; FAXF; FAZF; PLZP; Rog; TZFP; ZBTB32; ZNF538

UniProt ID: (Human) Q9Y2Y4, (Mouse) Q9JKD9

Entrez Gene ID: (Human) 27033, (Pig) 100627119, (Rat) 688845, (Mouse) 58206

Function(s)

DNA binding transcription corepressor activity protein binding zinc ion binding nucleic acid binding metal ion binding C2H2 zinc finger transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter interstrand cross-link repair DNA damage response, detection of DNA damage regulation of cytokine production hemopoiesis T cell proliferation negative regulation of nucleic acid-templated transcription transcription, DNA-templated regulation of transcription, DNA-templated

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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