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ZHX2 Antibody (CF810688) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZHX2 (RC208321, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ZHX2. (1. Positive lysates LY414909 (100 µg) and LC414909 (20 µg) can be purchased separately from OriGene.
Product Details
CF810688
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI9A5
Immunogen
Human recombinant protein fragment corresponding to amino acids 595-837 of human ZHX2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys(2)-His(2)-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AFP regulator 1; alpha fetoprotein regulation 1, adult; Alpha-fetoprotein regulator 1; Regulator of AFP; transcription factor ZHX2; Zinc finger and homeodomain protein 2; Zinc fingers and homeoboxes protein 2; zinc-fingers and homeoboxes 2
Gene Aliases: Afr-1; AFR1; KIAA0854; mKIAA0854; RAF; ZHX2
UniProt ID: (Human) Q9Y6X8, (Rat) Q80VX4, (Mouse) Q8C0C0
Entrez Gene ID: (Human) 22882, (Rat) 314988, (Mouse) 387609
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
mRNA catabolic process
somatic stem cell population maintenance
negative regulation of neuron differentiation
negative regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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