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Application
FIGURE: 1 / 1
ZNF265/ZIS Antibody (A301-030A) in WB
Detection of human ZNF265/ZIS by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF265/ZIS antibody (Product # A301-030A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. Efficient immunoprecipitation of ZNF265/ZIS was accomplished using rabbit anti-ZNF265/ZIS antibody A301-029A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds... View More
Product Details
A301-030A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 270 and the C-terminus (residue 320) of Human zinc-finger protein 265 (zinc-finger, splicing).
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZNF265 (Zinc finger protein 265), also known as ZRANB2 (Zinc finger Ran-binding domain-containing protein 2), ZIS, ZIS1 or ZIS2, is a 330 amino acid protein that belongs to the ZRANB2 family. Localized to the nucleus, ZNF265 functions as a splicing factor that is responsible for alternatively splicing Tra-2 beta (transformer-2 beta) transcripts and is thought to interfere with constitutive 5'-splice selection. ZNF265 contains two RanBP2-type zinc fingers through which it conveys its RNA-binding activity. Two isoforms, designated ZIS-1 and ZIS-2, are expressed due to alternative splicing events. Upon DNA damage, ZIS-2 may be phosphorylated by ATM or ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686J1831; DKFZp686N09117; Zinc finger protein 265; Zinc finger Ran-binding domain-containing protein 2; zinc finger, RAN-binding domain containing 2; Zinc finger, splicing; zinc-finger protein 265; zinc-finger, splicing
Gene Aliases: ZIS; ZIS1; ZIS2; ZNF265; ZRANB2
UniProt ID: (Human) O95218
Entrez Gene ID: (Human) 9406
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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