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Application
FIGURE: 1 / 3
ZNF50 Antibody (PA5-106774) in ICC/IF
Immunocytochemistry analysis of ZNF50 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ZNF50 polyclonal antibody (Product # PA5-106774) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106774
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ZSCAN22(Accession P10073), corresponding to amino acid residues A321-Q371.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854442
Product Specific Information
Antibody detects endogenous levels of total ZSCAN22.
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZSCAN22 (zinc finger and SCAN domain-containing protein 22), also known as HKR2 or ZNF50 (zinc finger protein 50), is a 491 amino acid nuclear protein. Belonging to the Krueppel C2 H2-type zinc-finger protein family, ZSCAN22 contains eight C2 H2-type zinc fingers and one SCAN box domain. ZSCAN22 may be involved in transcriptional regulation, and becomes phosphorylated upon DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI-Kruppel family member HKR2; Krueppel-related zinc finger protein 2; Protein HKR2; Zinc finger and SCAN domain-containing protein 22; Zinc finger protein 50; zinc finger protein 8-like
Gene Aliases: AW227621; D530006B18Rik; HKR2; ZNF50; ZSCAN22
UniProt ID: (Human) P10073
Entrez Gene ID: (Rat) 102553002, (Human) 342945, (Mouse) 232878
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