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Invitrogen

alpha-1 Antitrypsin Monoclonal Antibody (2B12)

Name: alpha-1 Antitrypsin Monoclonal Antibody (2B12)
Brand: Invitrogen
SKU: MA515521
Price: 552.0 USD

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2 Published Figures

2 References

View all (92) alpha-1 Antitrypsin antibodies

Datasheet

Protocols

Questions & Answers

Catalog # MA5-15521

Price (USD)

552.00

100 µL

Estimated availability date: 17-Apr-2025

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Datasheet

Protocols

Questions & Answers

Application

alpha-1 Antitrypsin Antibody in Western Blot (WB)

FIGURE: 1 / 6

alpha-1 Antitrypsin Antibody (MA5-15521) in WB

Western blot was performed using Anti-alpha-1 Antitrypsin Monoclonal Antibody (2B12)(Product # MA5-15521) and a ~55kDa band corresponding to alpha-1 Antitrypsin was observed across cell lines. Whole Cell Extract-TEB (30 µg lysate) of Hep G2 (Lane 1) and HT-29 (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:... View More

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Product Details

MA5-15521

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B12

Immunogen

Purified recombinant fragment of human AAT expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985373

Product Specific Information

MA5-15521 targets AAT in indirect ELISA, WB applications and shows reactivity with Human and mouse samples.

The MA5-15521 immunogen is purified recombinant fragment of human AAT expressed in E. Coli.

MA5-15521 detects AAT which has a predicted molecular weight of approximately 47kDa.

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.

Gene therapy

Janosz E,Hetzel M,Spielmann H,Tumpara S,Rossdam C,Schwabbauer M,Kloos D,von Kaisenberg C,Schambach A,Buettner FFR,Janciauskiene S,Lachmann N,Moritz T

Published figure using alpha-1 Antitrypsin monoclonal antibody (Product # MA5-15521) in Western Blot

Wed Sep 01 00:00:00 UTC 2021

Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase.

Scientific reports

Eggenschwiler R,Moslem M,Fráguas MS,Galla M,Papp O,Naujock M,Fonfara I,Gensch I,Wähner A,Beh-Pajooh A,Mussolino C,Tauscher M,Steinemann D,Wegner F,Petri S,Schambach A,Charpentier E,Cathomen T,Cantz T

MA515521 was used in western blot to optimize bi-allelic modification of transcriptionally silent loci in human pluripotent stem cells

Fri Dec 02 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: a-1 AT; a1-antitrypsin; AAT; alpha 1 antitrypsin; alpha-1 antitrypsin; Alpha-1 protease inhibitor; Alpha-1 protease inhibitor 1; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha-1-antitrypsin 1-1; alpha-1-antitrypsin 2; alpha-1-antitrypsin null; Digestive Zymogen; MGC23330; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; Protease Serine 1; serine (or cysteine) proteinase inhibitor, clade A, member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1a; Serine Protease 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1; Serpin A1a; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; TRP1; TRY1

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Gene Aliases: A1A; A1AT; AAT; Aat-2; Aat2; alpha1AT; Dom1; PI; PI1; PRO0684; PRO2209; PRO2275; SERPINA1; Serpina1a; Spi1-1; Spi1-3

UniProt ID: (Human) P01009, (Mouse) P07758

Entrez Gene ID: (Human) 5265, (Mouse) 20700

Function(s)

glycoprotein binding protease binding serine-type endopeptidase inhibitor activity protein binding identical protein binding endopeptidase inhibitor activity peptidase inhibitor activity protease inhibitor

Process(es)

platelet degranulation ER to Golgi vesicle-mediated transport acute-phase response blood coagulation negative regulation of endopeptidase activity COPII vesicle coating protein N-linked glycosylation negative regulation of peptidase activity response to cytokine response to peptide hormone

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