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            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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            • Primary Antibodies ›
            • alpha-2a Adrenergic Receptor Antibodies

            Invitrogen

            alpha-2a Adrenergic Receptor Polyclonal Antibody

            8 Published Figures
            15 References
            View all (11) alpha-2a Adrenergic Receptor antibodies
            Datasheet
            Protocols
            Questions & Answers
            Tech Support
            Catalog # PA1-048
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            Preis (EUR)
            548,00
            100 µL
             
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            Datasheet
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            Cite alpha-2a Adrenergic Receptor Polyclonal Antibody

            • Antibody Testing Data (1)
            • Published Figures (8)
            Application
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 9

            alpha-2a Adrenergic Receptor Antibody (PA1-048) in WB

            Western blot analysis was performed on whole cell extracts (30 µg lysate) of PC-3 (Lane 1), HEK-293 (Lane 2), T47D (Lane 3) and HEL 92.1.7 (lane 4). The blots were probed with Anti-alpha-2a Adrenergic Receptor Rabbit Polyclonal Antibody (Product # PA1-048, 1:250-1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 di... View More
            View Product

            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            alpha-2a Adrenergic Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))
            alpha-2a Adrenergic Receptor Antibody in Immunohistochemistry (IHC)
            alpha-2a Adrenergic Receptor Antibody in Immunohistochemistry (IHC)
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)
            alpha-2a Adrenergic Receptor Antibody in Western Blot (WB)

            Product Details

            PA1-048

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500
            View 6 publications 6 publications

            Immunohistochemistry (IHC)

            -
            View 6 publications 6 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:1,000
            -

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 2 publications 2 publications

            Immunocytochemistry (ICC/IF)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat

            Published species

            Human, Mouse, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.6 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS with 1mg/mL BSA

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2225243

            Product Specific Information

            PA1-048 detects alpha-2A adrenergic receptor (A2AAR) from human, rat and mouse tissues.

            PA1-048 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~45 kDa protein representing A2AAR from mouse kidney membrane preparations.

            The PA1-048 immunogen is a synthetic peptide corressponding to residues R(218) I Y Q I A K R R T R V P P S R R G(235) of the 3rd intracellular loop of human A2AAR. This sequence is completely conserved between human, mouse, rat, and porcine A2AAR.

            Target Information

            Alpha-2-adrenergic receptors (ADRA2A) are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. ADRA2A mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G protein. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: adrenergic receptor, alpha 2a; adrenergic, alpha-2A-, receptor; Alpha-2 adrenergic receptor subtype C10; alpha-2-adrenergic receptor, platelet type; Alpha-2A adrenergic receptor; Alpha-2A adrenoceptor; Alpha-2A adrenoreceptor; alpha-2AAR; alpha-2AAR subtype C10; Alpha-2D adrenergic receptor; alpha2A-adrenergic receptor; CA2-47; OTTHUMP00000058849; subtype alpha2-C10

            View more View less

            Gene Aliases: Adra-2; Adra-2a; ADRA2; ADRA2A; ADRA2R; ADRAR; alpha(2A)AR; alpha2-C10; alpha2A; alpha2A-AR; ALPHA2AAR; AW122659; CA2-47; RATRG20; RG20; ZNF32

            View more View less

            UniProt ID: (Human) P08913, (Mouse) Q01338, (Rat) P22909

            View more View less

            Entrez Gene ID: (Human) 150, (Mouse) 11551, (Rat) 25083

            View more View less

            Function(s)

            alpha2-adrenergic receptor activity protein binding protein kinase binding alpha-1B adrenergic receptor binding alpha-2C adrenergic receptor binding thioesterase binding heterotrimeric G-protein binding protein homodimerization activity protein heterodimerization activity epinephrine binding norepinephrine binding adrenergic receptor activity G-protein coupled receptor

            Process(es)

            positive regulation of cytokine production acute inflammatory response DNA replication movement of cell or subcellular component signal transduction G-protein coupled receptor signaling pathway negative regulation of adenylate cyclase activity Ras protein signal transduction Rho protein signal transduction female pregnancy positive regulation of cell proliferation negative regulation of norepinephrine secretion regulation of vasoconstriction actin cytoskeleton organization platelet activation positive regulation of cell migration negative regulation of cAMP biosynthetic process activation of protein kinase activity activation of protein kinase B activity negative regulation of epinephrine secretion cellular response to hormone stimulus epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway glucose homeostasis positive regulation of potassium ion transport positive regulation of MAP kinase activity positive regulation of epidermal growth factor-activated receptor activity positive regulation of vasodilation negative regulation of calcium ion-dependent exocytosis negative regulation of insulin secretion regulation of insulin secretion intestinal absorption thermoception negative regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis negative regulation of calcium ion transport negative regulation of insulin secretion involved in cellular response to glucose stimulus negative regulation of uterine smooth muscle contraction negative regulation of adrenergic receptor signaling pathway adenylate cyclase-activating adrenergic receptor signaling pathway adenylate cyclase-inhibiting adrenergic receptor signaling pathway phospholipase C-activating adrenergic receptor signaling pathway activation of MAPK activity by adrenergic receptor signaling pathway positive regulation of wound healing negative regulation of calcium ion transmembrane transporter activity cell-cell signaling fear response adrenergic receptor signaling pathway regulation of smooth muscle contraction
            It has to be done as per old AB suggested Products section.
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