Invitrogen

alpha Synuclein Monoclonal Antibody (Syn 211)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

19 References

View all (84) alpha Synuclein antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

alpha Synuclein Antibody in Western Blot (WB)

FIGURE: 1 / 11

alpha Synuclein Antibody (MA1-12874) in WB

Western blot was performed using Anti-alpha Synuclein Monoclonal Antibody, (Product # MA1-12874) and a ~14 kDa band corresponding to alpha Synuclein was observed in SH-SY5Y and HEK-293 cell lines but not in Hep G2 and A549 cell lines which are reported be expressing low to nil amounts of alpha Synuclein protein. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane1), HEK-293 (Lane 2), Hep G2 (Lane 3) and A549 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred... View More

alpha Synuclein Antibody in Immunocytochemistry (ICC/IF)

alpha Synuclein Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

alpha Synuclein Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-12874

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Syn 211

Immunogen

Recombinant human alpha-synuclein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C

Shipping conditions

Wet ice

RRID

AB_1070228

Product Specific Information

A suggested positive control for this product is SHSY-5Y cells. It will not cross-react with mouse or rat.

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinson's disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys.

EBioMedicine

Alarcón-Arís D,Pavia-Collado R,Miquel-Rio L,Coppola-Segovia V,Ferrés-Coy A,Ruiz-Bronchal E,Galofré M,Paz V,Campa L,Revilla R,Montefeltro A,Kordower JH,Vila M,Artigas F,Bortolozzi A

MA1-12874 was used in Western Blot, Immunohistochemistry (Frozen) to disrupt the α-synuclein mRNA transcription selectively in monoamine neurons of a PD-like mouse model and elderly nonhuman primates. This is to see if the inhibition of α-synuclein production in dopamine neurons and its accumulation in cortical/striatal projection areas may alleviate the early deficient dopamine function.

Tue Sep 01 00:00:00 EDT 2020

Anti-α-synuclein ASO delivered to monoamine neurons prevents α-synuclein accumulation in a Parkinson's disease-like mouse model and in monkeys.

EBioMedicine

Alarcón-Arís D,Pavia-Collado R,Miquel-Rio L,Coppola-Segovia V,Ferrés-Coy A,Ruiz-Bronchal E,Galofré M,Paz V,Campa L,Revilla R,Montefeltro A,Kordower JH,Vila M,Artigas F,Bortolozzi A

Tue Sep 01 00:00:00 EDT 2020

Multikinase Abl/DDR/Src Inhibition Produces Optimal Effects for Tyrosine Kinase Inhibition in Neurodegeneration.

Drugs in R&D

Fowler AJ,Hebron M,Missner AA,Wang R,Gao X,Kurd-Misto BT,Liu X,Moussa CE

MA1-12874 was used in Western Blotting to study the role of inhibition of Abelson tyrosine kinase as a therapeutic target in neurodegeneration.

Sat Jun 01 00:00:00 EDT 2019

Cortical phosphorylated α-Synuclein levels correlate with brain wave spectra in Parkinson's disease.

Movement disorders : official journal of the Movement Disorder Society

Caviness JN,Lue LF,Hentz JG,Schmitz CT,Adler CH,Shill HA,Sabbagh MN,Beach TG,Walker DG

MA1-12874 was used in western blot to analyze the correlation between brain wave spectra in Parkinson's disease and cortical phosphorylated alpha-synuclein levels

Fri Jul 01 00:00:00 EDT 2016

Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons.

Journal of neuroinflammation

López de Maturana R,Lang V,Zubiarrain A,Sousa A,Vázquez N,Gorostidi A,Águila J,López de Munain A,Rodríguez M,Sánchez-Pernaute R

MA112874 was used in immunocytochemistry to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease

Fri Nov 18 00:00:00 EST 2016

Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons.

Journal of neuroinflammation

López de Maturana R,Lang V,Zubiarrain A,Sousa A,Vázquez N,Gorostidi A,Águila J,López de Munain A,Rodríguez M,Sánchez-Pernaute R

MA112874 was used in immunocytochemistry to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease

Fri Nov 18 00:00:00 EST 2016

A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy.

Molecular neurodegeneration

Spencer B,Valera E,Rockenstein E,Trejo-Morales M,Adame A,Masliah E

MA1-12874 was used in western blot to reduce alpha-synuclein accumulation in a mouse model of multiple system atrophy by using a brain-targeted, modified neurosin (kallikrein-6)

Wed Sep 23 00:00:00 EDT 2015

Hypoestoxide reduces neuroinflammation and α-synuclein accumulation in a mouse model of Parkinson's disease.

Journal of neuroinflammation

Kim C,Ojo-Amaize E,Spencer B,Rockenstein E,Mante M,Desplats P,Wrasidlo W,Adame A,Nchekwube E,Oyemade O,Okogun J,Chan M,Cottam H,Masliah E

MA1-12874 was used in immunohistochemistry to study a mouse model of Parkinson's disease for hypoestoxide to reduce neuroinflammation and alpha-synuclein accumulation

Fri Dec 18 00:00:00 EST 2015

Lack of additive role of ageing in nigrostriatal neurodegeneration triggered by α-synuclein overexpression.

Acta neuropathologica communications

Bourdenx M,Dovero S,Engeln M,Bido S,Bastide MF,Dutheil N,Vollenweider I,Baud L,Piron C,Grouthier V,Boraud T,Porras G,Li Q,Baekelandt V,Scheller D,Michel A,Fernagut PO,Georges F,Courtine G,Bezard E,Dehay B

MA1-12874 was used in immunohistochemistry to study alpha-synuclein overexpression by lack of an additive role of aging in nigrostriatal neurodegeneration

Sat Jul 25 00:00:00 EDT 2015

Alpha-synuclein transfers from neurons to oligodendrocytes.

Glia

Reyes JF,Rey NL,Bousset L,Melki R,Brundin P,Angot E

MA1-12874 was used in immunohistochemistry to study the alpha-synuclein transfers from neurons to oligodendrocytes

Sat Mar 01 00:00:00 EST 2014

α-Synuclein impairs oligodendrocyte progenitor maturation in multiple system atrophy.

Neurobiology of aging

May VE,Ettle B,Poehler AM,Nuber S,Ubhi K,Rockenstein E,Winner B,Wegner M,Masliah E,Winkler J

MA1-12874 was used in immunohistochemistry to study multiple system atrophy and the role of alpha-synuclein in delaying oligodendrocyte progenitor maturation

Wed Oct 01 00:00:00 EDT 2014

Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.

Molecular and cellular neurosciences

Ettle B,Reiprich S,Deusser J,Schlachetzki JC,Xiang W,Prots I,Masliah E,Winner B,Wegner M,Winkler J

MA1-12874 was used in western blot to analyze the effect of human alpha-synuclein on early primary rat oligodendrocyte progenitor cell maturation

Mon Sep 01 00:00:00 EDT 2014

Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.

Molecular and cellular neurosciences

Ettle B,Reiprich S,Deusser J,Schlachetzki JC,Xiang W,Prots I,Masliah E,Winner B,Wegner M,Winkler J

MA1-12874 was used in western blot to analyze the effect of human alpha-synuclein on early primary rat oligodendrocyte progenitor cell maturation

Mon Sep 01 00:00:00 EDT 2014

Differential expression of alpha-synuclein in hippocampal neurons.

PloS one

Taguchi K,Watanabe Y,Tsujimura A,Tatebe H,Miyata S,Tokuda T,Mizuno T,Tanaka M

MA1-12874 was used in immunocytochemistry to study the hippocampal neuronal expression of alpha-synuclein under normal and pathological conditions

Mon Dec 22 00:00:00 EST 2014

α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity.

The Journal of biological chemistry

Chen RHC,Wislet-Gendebien S,Samuel F,Visanji NP,Zhang G,Marsilio D,Langman T,Fraser PE,Tandon A

MA1-12874 was used in western blot to study the role of Rab3a and synaptic activity in the membrane association of alpha-synuclein

Fri Mar 15 00:00:00 EDT 2013

α-synuclein phosphorylation and truncation are normal events in the adult human brain.

Neuroscience

Muntané G,Ferrer I,Martinez-Vicente M

MA1-12874 was used in western blot to study the constitutive truncation and phosphorylation of alpha-synuclein in normal human brain

Tue Jan 03 00:00:00 EST 2012

Neuropathology in mice expressing mouse alpha-synuclein.

PloS one

Rieker C,Dev KK,Lehnhoff K,Barbieri S,Ksiazek I,Kauffmann S,Danner S,Schell H,Boden C,Ruegg MA,Kahle PJ,van der Putten H,Shimshek DR

MA1-12874 was used in western blot to characterize mice that over express murine alpha-Synuclein

Thu Mar 01 00:00:00 EST 2012

A reassessment of the neuropathology of frontotemporal dementia linked to chromosome 3.

Journal of neuropathology and experimental neurology

Holm IE,Englund E,Mackenzie IR,Johannsen P,Isaacs AM

MA1-12874 was used in immunohistochemistry to re-evaluate the neuropathology of frontotemporal dementia linked to chromosome 3 in a Danish family

Mon Oct 01 00:00:00 EDT 2007

Abnormal alpha-synuclein solubility, aggregation and nitration in the frontal cortex in Pick's disease.

Neuroscience letters

Dalfó E,Martinez A,Muntané G,Ferrer I

MA1-12874 was used in western blot to study the distinct changes in the solubility, aggregation and tyrosine nitration of alpha-synuclein observed in the frontal cortex in Pick' disease

Mon May 29 00:00:00 EDT 2006

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alpha Synuclein Monoclonal Antibody (Syn 211)

alpha Synuclein Antibody (MA1-12874) in WB

Product Details

MA1-12874

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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