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beta-2 Defensin Antibody (PA5-103126) in ICC/IF
Immunofluorescent analysis of beta-2 Defensin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and beta-2 Defensin polyclonal antibody (Product # PA5-103126) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Ale... View More
Product Details
PA5-103126
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DEFB4A; DEFB4B(Accession O15263), corresponding to amino acid residues V29-P64.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852496
Product Specific Information
Antibody detects endogenous levels of total Defensin beta-2.
Target Information
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, six human beta-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; Defensin beta 2; Defensin beta 4A; Defensin beta2; Defensin, beta 2; defensin, beta 4; defensin, beta 4A; Defensin-beta2; SAP1; Skin-antimicrobial peptide 1
Gene Aliases: BD-2; DEFB-2; DEFB102; DEFB2; DEFB4; DEFB4A; DEFB4B; HBD-2; SAP1
UniProt ID: (Human) O15263
Entrez Gene ID: (Human) 1673
Performance Guarantee
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