Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
beta-2 Microglobulin Monoclonal Antibody (B2M-01), Alexa Fluor™ 647
Application
FIGURE: 1 / 5
beta-2 Microglobulin Antibody (MA5-18119) in ICC/IF
Immunocytochemistry analysis of human β2-microglobulin-transfected canine cells labeled A) with B2M-01 Monoclonal antibody Alexa Fluor® 647 conjugate (Product # MA5-18119) and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2 µg/mL.
Product Details
MA5-18119
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
B2M-01
Immunogen
Purified human beta2-microglobulin
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539493
Product Specific Information
This antibody reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
It will not cross-react with mouse, rabbit, canine, chicken or bovine.
Target Information
Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Video Player is loading.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: B-F; B2-microglobulin; Beta 2 microglobin; Beta 2 microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta2 microglobulin; MHC Class I; microglobulin beta-2; Orla-B2m
Gene Aliases: B2M; CDABP0092; HDCMA22P; IMD43
UniProt ID: (Human) P61769
Entrez Gene ID: (Human) 567
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support