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Application
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beta-3 Tubulin Antibody (MA1-9587) in ICC/IF
Immunofluorescence analysis of beta-3 Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta-3 Tubulin (5G8) Mouse Monoclonal Antibody (Product # MA1-9587) at 2µg/mLin 0.1% BSA and incubated fo... View More
Product Details
MA1-9587
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5G8
Immunogen
Synthetic peptide corresponding to the C-terminus of human beta III tubulin.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2241264
Product Specific Information
MA1-9587 detects Beta III Tubulin in human, mouse and rat samples.
MA1-9587 has been successfully used in Western blot, ELISA, immunohistochemistry, and immunocytochemistry procedures. By Western blot, MA1-9587 detects a 50 kDa protein representing Beta III Tubulin.
The MA1-9587 immunogen is a synthetic peptide corresponding to the C-terminus of human beta III tubulin.
Store at 2-8C for one week. For long term storage, store at -20C.
Target Information
The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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