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            • Primary Antibodies ›
            • beta Actin Antibodies

            Proteintech

            beta Actin Polyclonal Antibody

            2,725 References
            View all (96) beta Actin antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite beta Actin Polyclonal Antibody

            • Antibody Testing Data (25)
            Application
            beta Actin Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 25

            beta Actin Antibody (20536-1-AP) in ICC/IF

            Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (Product # 20536-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
            View Product

            beta Actin Antibody in Immunocytochemistry (ICC/IF)
            beta Actin Antibody in Immunocytochemistry (ICC/IF)
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Western Blot (WB)
            beta Actin Antibody in Flow Cytometry (Flow)

            Product Details

            20536-1-AP

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:5,000-1:15,000
            View 2,612 publications 2,612 publications

            Immunohistochemistry (IHC)

            -
            View 3 publications 3 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            1:50-1:500
            -

            Immunocytochemistry (ICC/IF)

            1:10-1:100
            View 7 publications 7 publications

            Flow Cytometry (Flow)

            Assay-dependent
            -

            Immunoprecipitation (IP)

            -
            View 6 publications 6 publications

            Miscellaneous PubMed (Misc)

            -
            View 41 publications 41 publications

            Not applicable (N/A)

            -
            View 56 publications 56 publications
            Product Specifications

            Species Reactivity

            Dog, Human, Mouse, Non-human primate, Rat

            Published species

            Goat, Hamster, Human, Mammal, Mouse, Pig, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            beta Actin Fusion Protein Ag14521 (1-50 aa encoded by BC002409)
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.43 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.3, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Wet ice (domestic); Dry ice (international)

            Product Specific Information

            This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.

            Immunogen sequence: MDDDIAALV VDNGSGMCKA GFAGDDAPRA VFPSIVGRPR HQGVMVGMGQ K (1-50 aa encoded by BC002409)

            Target Information

            Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: actin, beta; Actin, cytoplasmic 1; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; PS1TP5-binding protein 1

            View more View less

            Gene Aliases: ACTB; Actx; beta-actin; BRWS1; E430023M04Rik; PS1TP5BP1

            View more View less

            UniProt ID: (Human) P60709, (Dog) O18840, (Rat) P60711, (Mouse) P60710

            View more View less

            Entrez Gene ID: (Human) 60, (Dog) 403580, (Rat) 81822, (Mouse) 11461

            View more View less

            Function(s)

            RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding structural constituent of cytoskeleton protein binding ATP binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding protein kinase binding actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

            Process(es)

            retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation postsynaptic actin cytoskeleton organization axonogenesis retina development in camera-type eye cellular response to electrical stimulus
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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