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            • Primary Antibodies ›
            • beta Catenin Antibodies

            Invitrogen

            beta Catenin Polyclonal Antibody

            Advanced Verification
            4 Published Figures
            6 References
            View all (241) beta Catenin antibodies
            Datasheet
            Protocols
            Questions & Answers
            Datasheet
            Protocols
            Questions & Answers

            Cite beta Catenin Polyclonal Antibody

            • Antibody Testing Data (22)
            • Published Figures (4)
            • Advanced Verification (1)
            Application
            beta Catenin Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 27

            beta Catenin Antibody (PA5-77934) in ICC/IF

            beta Catenin Polyclonal Antibody detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin Polyclonal Antibody (Product # PA5-77934) diluted at 1:500. Blue: Hoechst 33342 staining.
            View Product

            beta Catenin Antibody in Immunocytochemistry (ICC/IF)
            beta Catenin Antibody in Immunocytochemistry (ICC/IF)
            beta Catenin Antibody in Immunocytochemistry (ICC/IF)
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Flow Cytometry (Flow)
            beta Catenin Antibody in Immunoprecipitation (IP)
            beta Catenin Antibody in ChIP Assay (ChIP)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody in Immunocytochemistry (ICC/IF)
            beta Catenin Antibody in Western Blot (WB)
            beta Catenin Antibody

            Product Details

            PA5-77934

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:20,000
            View 4 publications 4 publications

            Immunohistochemistry (IHC)

            -
            View 1 publication 1 publication

            Immunohistochemistry (Paraffin) (IHC (P))

            1:100-1:1,000
            -

            Immunohistochemistry (Frozen) (IHC (F))

            Assay-dependent
            -

            Immunocytochemistry (ICC/IF)

            1:100-1:1,000
            View 1 publication 1 publication

            Flow Cytometry (Flow)

            1:50-1:200
            -

            Immunoprecipitation (IP)

            1:50-1:100
            -

            ChIP assay (ChIP)

            Assay-Dependent
            -
            Product Specifications

            Species Reactivity

            Human, Mouse, Rabbit, Rat

            Published species

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.13 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7, with 1% BSA, 20% glycerol

            Contains

            0.025% ProClin 300

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2735522

            Product Specific Information

            Positive Control: HeLa, A549, H1299, HCT116, PC-12, Mouse brain, human fibroblast, LX2

            Predicted Reactivity: Zebrafish (95%), Xenopus laevis (97%), Dog (99%), Pig (99%), Chicken (97%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%)

            Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

            Target Information

            Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: Bcatenin; beta 1 88kDa; Beta-catenin; Betacatenin; C20orf33; Cadherin associated protein; catenin; catenin (cadherin associated protein), beta 1, 88kDa; catenin (cadherin-associated protein), beta 1; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin b 1; Catenin b1; catenin beta; Catenin beta-1; Catenin beta1; Catenin β 1; Catenin β1; CTNB1; dJ633O20.1; DKFZp686D02253; FLJ25606; FLJ37923; NAP; NYD-SP19; OTTHUMP00000209289; P14L; PP8304; RP5-1118M15.1; β catenin; βcatenin

            View more View less

            Gene Aliases: armadillo; Bfc; Catnb; CTNNB; CTNNB1; Mesc; MRD19; OK/SW-cl.35; PRO2286

            View more View less

            UniProt ID: (Human) P35222, (Mouse) Q02248, (Rat) Q9WU82

            View more View less

            Entrez Gene ID: (Rabbit) 100125985, (Human) 1499, (Mouse) 12387, (Rat) 84353

            View more View less

            Function(s)

            RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity signal transducer activity protein binding protein C-terminus binding transcription factor binding enzyme binding kinase binding protein kinase binding protein phosphatase binding estrogen receptor binding protein complex binding ionotropic glutamate receptor binding nuclear hormone receptor binding transcription regulatory region DNA binding ion channel binding alpha-catenin binding cadherin binding SMAD binding androgen receptor binding nitric-oxide synthase binding I-SMAD binding repressing transcription factor binding cadherin binding involved in cell-cell adhesion euchromatin binding DNA binding chromatin binding R-SMAD binding

            Process(es)

            negative regulation of transcription from RNA polymerase II promoter embryonic axis specification cell morphogenesis involved in differentiation skeletal system development patterning of blood vessels vasculogenesis branching involved in ureteric bud morphogenesis in utero embryonic development gastrulation with mouth forming second endoderm formation cell fate specification cell fate determination endodermal cell fate commitment neuron migration kidney development neural plate development vasculature development positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation lens morphogenesis in camera-type eye ventricular compact myocardium morphogenesis regulation of secondary heart field cardioblast proliferation mesenchymal to epithelial transition involved in metanephros morphogenesis metanephros morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell adhesion cell-matrix adhesion chemical synaptic transmission ectoderm development nervous system development glial cell fate determination heart development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation anterior/posterior axis specification dorsal/ventral axis specification dorsal/ventral pattern formation proximal/distal pattern formation cellular process regulation of gene expression positive regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of heparan sulfate proteoglycan biosynthetic process Schwann cell proliferation Wnt signaling pathway morphogenesis of embryonic epithelium single organismal cell-cell adhesion stem cell population maintenance layer formation in cerebral cortex central nervous system vasculogenesis hair cycle process hemopoiesis cell differentiation T cell differentiation osteoclast differentiation lung development male genitalia development regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation forebrain development regulation of centriole-centriole cohesion pancreas development hair follicle morphogenesis regulation of myelination positive regulation of telomere maintenance via telomerase negative regulation of chondrocyte differentiation response to estradiol T cell differentiation in thymus negative regulation of protein sumoylation response to cytokine adherens junction organization adherens junction assembly protein localization to cell surface cellular protein localization embryonic heart tube development genitalia morphogenesis embryonic forelimb morphogenesis embryonic hindlimb morphogenesis embryonic skeletal limb joint morphogenesis regulation of cell proliferation regulation of T cell proliferation odontogenesis of dentin-containing tooth regulation of cell fate specification embryonic digit morphogenesis regulation of apoptotic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade skin development response to estrogen canonical Wnt signaling pathway involved in positive regulation of epithelial to mesenchymal transition canonical Wnt signaling pathway involved in negative regulation of apoptotic process bone resorption regulation of cell differentiation negative regulation of cell differentiation positive regulation of endothelial cell differentiation regulation of osteoblast differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of osteoclast differentiation positive regulation of fibroblast growth factor receptor signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of mitotic cell cycle, embryonic chromatin-mediated maintenance of transcription cell maturation synaptic vesicle transport animal organ development thymus development oocyte development embryonic foregut morphogenesis regulation of smooth muscle cell proliferation negative regulation of oligodendrocyte differentiation synapse organization smooth muscle cell differentiation protein heterooligomerization positive regulation of telomerase activity cardiac muscle cell proliferation oviduct development canonical Wnt signaling pathway limb development trachea morphogenesis trachea formation epithelial tube branching involved in lung morphogenesis lung cell differentiation lung-associated mesenchyme development mesenchyme development lung induction epithelial cell differentiation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development hair follicle placode formation mesenchymal cell proliferation involved in lung development cardiac vascular smooth muscle cell differentiation coronary artery morphogenesis epicardium-derived cardiac vascular smooth muscle cell differentiation positive regulation of branching involved in lung morphogenesis endothelial tube morphogenesis fungiform papilla formation canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation sympathetic ganglion development regulation of centromeric sister chromatid cohesion cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to organic cyclic compound cellular response to indole-3-methanol renal system development renal vesicle formation renal inner medulla development renal outer medulla development nephron tubule formation mesenchyme morphogenesis regulation of nephron tubule epithelial cell differentiation regulation of calcium ion import negative regulation of neuron death cellular response to insulin-like growth factor stimulus regulation of protein localization to cell surface positive regulation of determination of dorsal identity negative regulation of apoptotic signaling pathway liver development midgut development response to hormone response to organic cyclic compound response to drug myoblast differentiation response to cadmium ion epithelial to mesenchymal transition negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis Wnt signaling pathway, calcium modulating pathway response to activity androgen receptor signaling pathway hindbrain development positive regulation of type I interferon production hair cell differentiation catenin import into nucleus proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process cellular response to fibroblast growth factor stimulus regulation of angiogenesis regulation of fibroblast proliferation positive regulation of skeletal muscle tissue development embryonic cranial skeleton morphogenesis regulation of neurogenesis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of muscle cell differentiation positive regulation of histone H3-K4 methylation cranial ganglion development cellular response to lithium ion positive regulation of chromatin-mediated maintenance of transcription regulation of euchromatin binding regulation of core promoter binding beta-catenin-TCF complex assembly beta-catenin destruction complex disassembly midbrain dopaminergic neuron differentiation canonical Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation embryonic brain development dorsal root ganglion development positive regulation of DNA-templated transcription, initiation
            It has to be done as per old AB suggested Products section.
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