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Bethyl Laboratories

c-Myb Polyclonal Antibody

View all (30) Myb antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

c-Myb Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

c-Myb Antibody (A304-135A) in IHC (P)

Detection of human c-Myb by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti-c-Myb antibody (Product # A304-135A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) and 1:1,000 (1 µg/mL). Detection: DAB.

c-Myb Antibody in Immunoprecipitation (IP)

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Product Details

A304-135A

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

1:1,000-1:5,000

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

Immunoprecipitation (IP)

2-10 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1 to 50 of human V-myb Myeloblastosis Viral Oncogene Homolog.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; gag-myb protein; myb proto-oncogene protein; myeloblastosis proto-oncogene product; oncogene AMV; Proto-oncogene c-Myb; RP1-32B1.1; Transcriptional activator Myb; tumor-specific myb protein; v-myb avian myeloblastosis viral oncogene homolog

Gene Aliases: AI550390; c-myb; c-myb_CDS; Cmyb; efg; M16449; MYB

UniProt ID: (Human) P10242, (Mouse) P06876

Entrez Gene ID: (Human) 4602, (Mouse) 17863

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II transcription factor recruiting protein binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding WD40-repeat domain binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle chromatin remodeling regulation of transcription, DNA-templated transcription from RNA polymerase II promoter positive regulation of T-helper cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of histone H3-K4 methylation positive regulation of histone H3-K9 methylation G1/S transition of mitotic cell cycle in utero embryonic development transcription, DNA-templated calcium ion transport regulation of gene expression B cell differentiation thymus development embryonic digestive tract development homeostasis of number of cells cellular response to interleukin-6

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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