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Bethyl Laboratories
eIF2alpha/EIF2S1 Polyclonal Antibody
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Application
FIGURE: 1 / 2
eIF2alpha/EIF2S1 Antibody (A300-721A) in IHC (P)
Detection of human and mouse eIF2alpha/EIF2S1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF2alpha/EIF2S1 (Product # A300-721A) (Lot 1) used at a dilution of 1:1,000 (0.2 µg/mL) and 1:200 (1 µg/mL). Detection: DAB.
Product Details
A300-721A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 2, subunit 1 alpha, 35kDa.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-alpha; eIF-2A; eIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A
Gene Aliases: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1
UniProt ID: (Human) P05198, (Mouse) Q6ZWX6
Entrez Gene ID: (Human) 1965, (Mouse) 13665
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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