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Invitrogen
eIF4A1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
eIF4A1 Antibody (PA5-34726) in ICC/IF
Immunofluorescence analysis of eIF4A1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with eIF4A1 Polyclonal Antibody (Product # PA5-34726) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugat... View More
Product Details
PA5-34726
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 295 of EIF4A1 (Uniprot ID#P60842)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552078
Product Specific Information
Recommended positive controls: HepG2.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (96%), Dog (100%), Pig (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP-dependent RNA helicase eIF4A-1; eIF-4A-I; eIF4A-I; Eukaryotic initiation factor 4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; initiation factor eIF-4A long form
Gene Aliases: BM-010; DDX2A; EIF-4A; eIF-4A-I; Eif4; EIF4A; eIF4A-I; EIF4A1
UniProt ID: (Human) P60842, (Mouse) P60843
Entrez Gene ID: (Human) 1973, (Mouse) 13681, (Rat) 287436
RNA cap binding
double-stranded RNA binding
mRNA binding
translation initiation factor activity
ATP-dependent RNA helicase activity
helicase activity
protein binding
ATP binding
translation factor activity, RNA binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
hydrolase activity
molecular_function
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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