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eIF4G Antibody (PA5-96502) in IHC (P)
Immunohistochemistry analysis of eIF4G in paraffin-embedded human breast cancer using. Samples were incubated with eIF4G Polyclonal antibody (Product # PA5-96502) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
PA5-96502
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_8865533)
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808304
Product Specific Information
Immunogen sequence: PEELLNGAPS PPAVDLSPVS EPEEQAKEVT ASMAPPTIPS ATPATAPSAT SPAQEEEMEE EEEEEEGEAG EAGEAESEKG GEELLPPEST PIPANLSQNL E; Positive Samples: MCF7, U251, BT474, HeLa
Target Information
The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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