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Invitrogen
gag-pol Polyclonal Antibody
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Application
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gag-pol Antibody (PA5-144418) in WB
Western blot analysis of gag-pol in recombinant protein using a dilution of 1:2,000. Samples were incubated with gag-pol polyclonal antibody (Product # PA5-144418). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 23.2 kDa. Observed band size: 26 kDa.
Product Details
PA5-144418
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Moloney murine leukemia virus (isolate Shinnick) (MoMLV) POL protein (1444-1738aa)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.76 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091706
Target Information
[Gag-Pol polyprotein]: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag-Pol, or to Gag-Pol binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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