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p130 Antibody (PA1-32114) in IHC (P)
Immunohistochemical staining of mouse tissue using p130 Polyclonal Antibody (Product # PA1-32114). The staining shows the location of pRb2/p130 in developing mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37&#deg;C. All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in pho... View More
Product Details
PA1-32114
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Rb2 (p130) peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2175586
Product Specific Information
Store as a concentrated solution. Centrifuge briefly prior to opening vial.
Target Information
RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and (in particular) that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C leading to epigenetic transcriptional repression. It controls histone H4 'Lys20' trimethylation. It probably also acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa retinoblastoma-associated protein; FLJ26459; P130; PPAR-alpha-interacting complex protein 128; PRB2; PRIC128; rb2 p130; RBR-2; retinoblastoma-like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2
Gene Aliases: P130; PRB2; RB2; RBL2; RBR-2
UniProt ID: (Human) Q08999, (Mouse) Q64700, (Rat) O55081
Entrez Gene ID: (Human) 5934, (Mouse) 19651, (Rat) 81758
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
negative regulation of gene expression
covalent chromatin modification
regulation of lipid kinase activity
regulation of transcription, DNA-templated
cell cycle
chromatin modification
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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