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p70 S6 kinase alpha Antibody (BS-6370R) in WB
Lane 1: MCF-7 cell lysates; Lane 2: U-2os cell lysates; Lane 3: Molt-4 cell lysates probed with p70 S6 kinase alpha Polyclonal Antibody, Unconjugated (bs-6370R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Product Details
BS-6370R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha, amino acids 251-350.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 70 kDa ribosomal protein S6 kinase 1; EC 2.7.11.1; kinase p70S6K; KS6B1; p70 p70-S6K; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha; p70 S6K-alpha; p70 S6KA; p70(S6K)-alpha; p70-S6K 1; P70S6K; p70S6K-alpha; P70S6K1; p80-S6K 1; p85 p85S6K; Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6 kinase; S6K; S6K-beta-1; S6K1; serine/threonine kinase; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A
Gene Aliases: 2610318I15Rik; 4732464A07Rik; 70kDa; AA959758; AI256796; AI314060; p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; p70/85s6k; p70s6k; PS6K; RPS6KB1; S6K; S6K-beta-1; S6K1; STK14A
UniProt ID: (Human) P23443, (Bovine) Q6TJY3, (Mouse) Q8BSK8, (Rat) P67999
Entrez Gene ID: (Human) 6198, (Bovine) 404181, (Mouse) 72508, (Rat) 83840
protein kinase activity
ribosomal protein S6 kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
PDZ domain binding
peptide binding
identical protein binding
protein phosphatase 2A binding
nucleotide binding
protein serine/threonine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein modifying enzyme
G1/S transition of mitotic cell cycle
behavioral fear response
skeletal muscle contraction
protein phosphorylation
apoptotic process
signal transduction
germ cell development
aging
response to nutrient
long-term memory
response to heat
response to wounding
response to mechanical stimulus
response to toxic substance
response to glucose
skeletal muscle atrophy
response to electrical stimulus involved in regulation of muscle adaptation
positive regulation of smooth muscle cell migration
cell migration
TOR signaling
response to lipopolysaccharide
response to insulin
cellular response to hormone stimulus
response to testosterone
response to glucagon
response to tumor necrosis factor
response to drug
negative regulation of apoptotic process
response to leucine
protein kinase B signaling
response to ethanol
positive regulation of translation
positive regulation of mitotic cell cycle
positive regulation of translational initiation
regulation of glucose import
negative regulation of insulin receptor signaling pathway
phosphatidylinositol-mediated signaling
positive regulation of skeletal muscle tissue growth
positive regulation of smooth muscle cell proliferation
response to glucocorticoid
cellular response to growth factor stimulus
cellular response to organic cyclic compound
negative regulation of extrinsic apoptotic signaling pathway
regulation of translation
cell cycle
phosphatidylinositol 3-kinase signaling
peptidyl-serine phosphorylation
cellular response to insulin stimulus
long-chain fatty acid import
phosphorylation
response to organic substance
response to organonitrogen compound
response to organic cyclic compound
response to amino acid
response to peptide hormone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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