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Invitrogen
phoP Polyclonal Antibody
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Application
FIGURE: 1 / 1
phoP Antibody (PA5-144500) in WB
Western blot analysis of phoP in recombinant protein using a dilution of 1:2,000. Samples were incubated with phoP polyclonal antibody (Product # PA5-144500). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 30.5 kDa. Observed band size: 37 kDa.
Product Details
PA5-144500
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP) (1-223AA)
Conjugate
Unconjugated
Form
Liquid
Concentration
4.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3092373
Target Information
Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg(2+) environments and the control of acid resistance genes. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG (By similarity). Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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