PowerTrack SYBR Green Master Mix

Simplify your workflow with minimal primer optimization and reduced pipetting errors

Applied Biosystems PowerTrack SYBR Green Master Mix is a preformulated, optimized, universal 2X master mix for real-time PCR. Building on over 25 years of innovation and product excellence in real-time PCR (qPCR), this master mix is designed for superior performance and ease-of-use including an optional two-color tracking dye system to indicate where pipetting has occurred. Ideal for the most common real-time PCR applications, PowerTrack SYBR Green Master Mix delivers broad primer Tm , contamination control, and superior specificity and reproducibility over a wide dynamic range.

product image of Applied Biosystems PowerTrack SYBR Green Master Mix

Features

Features of PowerTrack SYBR Green Master Mixes include:

Two-color tracking dye system for pipetting quality control

Designed to help avoid pipetting errors by turning green when the sample is added to the master mix. Extensive testing has verified that the tracking dye does not reduce data quality.

See how it works

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      Broad primer compatibility

      Broad primer Tm and primer concentration compatibility allows flexibility in qPCR reaction setup with minimal optimization

      Contamination control

      Inclusion of UNG and dUTP allows any previously amplified PCR products to be degraded and helps prevent contamination of subsequent qPCR reactions.

      Superior specificity and reproducibility

      Antibody-mediated hot-start mechanism provides tight control over Taq enzyme activation and helps prevent early activity of the polymerase at low temperatures, allowing for tighter reproducibility.

      Broad instrument compatibility

      Can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments as well as many other manufacturers’ instruments.

      Sample data

      Power through traditionally difficult targets

      PowerTrack SYBR Green Master Mix delivers accurate results over a wide dynamic range of concentrations as shown by tight curves between replicates and superior PCR efficiency.

      Quadruplicate reactions show high accuracy between replicates and superior qPCR efficiency

      Linear dynamic range. Amplification curves were obtained for the PGK1 gene over a 5-log dilution series of UHR cDNA.  Reactions were run in quadruplicate on the Applied Biosystems QuantStudio 5 Real-Time PCR System with the 384-well block according to protocol.  10-µL reactions were run in quadruplicate using 60ºC annealing primer Tm. 

      Data figure showing quadruplicate reactions show high accuracy between replicates and superior qPCR efficiency

      Superior specificity

      The high specificity enabled by PowerTrack SYBR Green Master Mix allows you to spend less time optimizing and redesigning primers to get high quality data.

      Target specificity

      In an evaluation of 24 different primer sets used with PowerTrack SYBR Green Master Mix, a single melt curve was obtained in 100% of reactions. By comparison, nonspecific amplification was observed for some of the same targets with several master mixes from other suppliers, as shown by multiple peaks in the melt curves.

      Data image showing target specificity

      Excellent reproducibility

      Tighter reproducibility allows for greater statistical significance when analyzing low-abundance transcripts and smaller fold changes. PowerTrack SYBR Green Master Mix demonstrated excellent reproducibility over a wide dynamic range when tested with a variety of targets and RT kits.

      Reproducibility of data

      Six assays (PGK1, ARL1, SNF8, DF, GAPDH, and Corf1 19) were run in quadruplicate with UHR cDNA generated from four different reverse transcription (RT) kits (SuperScript IV VILO, High Capacity RNA to cDNA, iScript cDNA synthesis, and Quantitect RT kits) run with 6-fold dilution series with 400 nM primer concentration on the QuantStudio 5 Flex Real-Time PCR System according to each master mix manufacturer’s recommended protocol.

      Data figure illustrating reproducibility of data

      Complete your workflow

      Maximize real-time PCR results by pairing PowerTrack SYBR Green Master Mix with either SuperScript IV VILO reverse transcriptase for superior cDNA synthesis or SuperScript IV CellsDirect cDNA synthesis kit to go from mammalian cells all the way to first-strand cDNA synthesis without having to isolate RNA.  These best-in-class reagents help accelerate your research by offering accurate data quickly.

       

      Complete your qPCR workflow by pairing PowerTrack SYBR Green Master Mix with sequence detection primers: high-quality, unlabeled oligos for use with SYBR dye in all your real-time PCR research applications. Sequence detection primers are synthesized using the same raw materials and carefully controlled processes as our TaqMan probes, ensuring consistency and quality in every batch.

      Ordering information

      For Research Use Only. Not for use in diagnostic procedures.