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Simplify your workflow with minimal primer optimization and reduced pipetting errors
Applied Biosystems PowerTrack SYBR Green Master Mix is a preformulated, optimized, universal 2X master mix for real-time PCR. Building on over 25 years of innovation and product excellence in real-time PCR (qPCR), this master mix is designed for superior performance and ease-of-use including an optional two-color tracking dye system to indicate where pipetting has occurred. Ideal for the most common real-time PCR applications, PowerTrack SYBR Green Master Mix delivers broad primer Tm , contamination control, and superior specificity and reproducibility over a wide dynamic range.
Features
Features of PowerTrack SYBR Green Master Mixes include:
Two-color tracking dye system for pipetting quality control
Designed to help avoid pipetting errors by turning green when the sample is added to the master mix. Extensive testing has verified that the tracking dye does not reduce data quality.
See how it works
Broad primer compatibility
Broad primer Tm and primer concentration compatibility allows flexibility in qPCR reaction setup with minimal optimization
Contamination control
Inclusion of UNG and dUTP allows any previously amplified PCR products to be degraded and helps prevent contamination of subsequent qPCR reactions.
Superior specificity and reproducibility
Antibody-mediated hot-start mechanism provides tight control over Taq enzyme activation and helps prevent early activity of the polymerase at low temperatures, allowing for tighter reproducibility.
Broad instrument compatibility
Can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments as well as many other manufacturers’ instruments.
Sample data
Power through traditionally difficult targets
PowerTrack SYBR Green Master Mix delivers accurate results over a wide dynamic range of concentrations as shown by tight curves between replicates and superior PCR efficiency.
Superior specificity
The high specificity enabled by PowerTrack SYBR Green Master Mix allows you to spend less time optimizing and redesigning primers to get high quality data.
Excellent reproducibility
Tighter reproducibility allows for greater statistical significance when analyzing low-abundance transcripts and smaller fold changes. PowerTrack SYBR Green Master Mix demonstrated excellent reproducibility over a wide dynamic range when tested with a variety of targets and RT kits.
Complete your workflow
Maximize real-time PCR results by pairing PowerTrack SYBR Green Master Mix with either SuperScript IV VILO reverse transcriptase for superior cDNA synthesis or SuperScript IV CellsDirect cDNA synthesis kit to go from mammalian cells all the way to first-strand cDNA synthesis without having to isolate RNA. These best-in-class reagents help accelerate your research by offering accurate data quickly.
Complete your qPCR workflow by pairing PowerTrack SYBR Green Master Mix with sequence detection primers: high-quality, unlabeled oligos for use with SYBR dye in all your real-time PCR research applications. Sequence detection primers are synthesized using the same raw materials and carefully controlled processes as our TaqMan probes, ensuring consistency and quality in every batch.
Ordering information
For Research Use Only. Not for use in diagnostic procedures.