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Panel Design- Backbone of Flow Cytometry
Artists can have all the right brushes, but it’s no good without the right canvas to paint on. Similarly, you may have the best flow cytometer and dyes, but without the optimum panel design it may not yield best results. Building a panel is complex. It is not like a simple addition and mixing of conjugated antibodies and dyes with the sample. It is a complex permutation and combination to achieve the cleanest answer to the scientific question under investigation. There are many factors to be considered while building a panel. For example, fluorochrome brightness, compensation issues and spillovers, selecting appropriate controls are some of them. This blog can be a guide for your flow cytometry panel design.
Good panel design tools should give scientists the option to incorporate various dyes. It should also provide a platform to evaluate, analyze and forecast the complexities that may arise as a result of mixing different dye combinations. This makes the process easier for your use, improving time efficiency, allowing customization, and enabling panel expansions to maximize the panel’s potential. In a way, panel design is the backbone of flow cytometry experiments.
Tools For Flow Cytometry Panel Design
Flow cytometry functions much like the automobile assembly line. Processing cells instead of mass-producing cars, immunologists place single cells into a suspension, run them past a laser beam, and then count and sort reactions of each cell for further study, processing thousands in a matter of seconds. With the right tool at their disposal, this speeds up the counting and sorting of individual cells while collecting quality data. The Invitrogen™ Flow Cytometry Panel Builder addresses this need, making it ideal for both conventional and spectral flow cytometers. The panel builder’s flexibility and intuitiveness give scientists an edge in this crucial field. Moreover, it breaks down the complex process in simple steps along with resources for tips and tricks.
Simple and highly customizable, the online design tool allows researchers to configure their own flow cytometry panel. Thus, it lets researchers conduct complex, high parameter experiments best suited to your needs. Don’t leave anything to imagination; the panel builder allows you to select antibodies via auto or manual mode using spectral visualization. There are considerable resources available with the panel builder. The guides help in reducing spill over and selecting appropriate dyes for biomarkers with varying brightness as per need. As if these features were not enough, you can also get free support from experts to build your panel. Although there are many other resources and tools available at fingertips on internet-based search engines, out there, only we offer all these advantages in one place.
Good Panel Paves the Way for Discovery
The time- and effort-saving benefits of flow cytometry are crystal clear. Flow cytometry instruments can sort and analyze thousands of cells per minute, and the Flow Cytometry Panel Builder simplifies this process. By facilitating fast and optimum panel design, the panel builder frees up your time. It lets you focus on what matters most – research and discovery.
Learn more about the solution in this blog: Invitrogen Flow Cytometry Panel Builder
Resources and Solutions for Flow Cytometry
- Flow Cytometry Panel Builder – this online tool helps you design an effective reagent panel for your specific application on your conventional or spectral flow cytometer
- Flow Cytometry Protocols Handbook – protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping and data analysis strategies
- Immunology at Work Resource Center – practical guidance and protocols to help you get started quickly and confidently
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