Sterilization in the lab: Steri-Run for CO2 incubators

Knowing that your cell culture incubator is free from potentially contaminating microorganisms after a thorough clean is very important, but manual cleaning and disinfection can be a daunting task. An automated sterilization cycle can save time and offers confidence in the integrity of your work.

Several Thermo Scientific CO₂ incubator models feature proven high temperature sterilization methods. Both our 140 ˚ C Steri-Cycle and our 180 ˚ C Steri-Run sterilization processes meet requirements in the U.S. Pharmacopeia (USP and the European Union Pharmacopeia (EUP) for statistical proof of sterility using the overkill method, which requires a 12-log elimination of specific heat-resistant microorganisms. These standards also require that the hot air be kept continuously circulated “using a fan or blower”. Proof of success of the 140 ˚ C cycle is found in one application note. Our 180 ˚ C Steri-Run cycle evidence is found in another. In both cases, the tests were conducted by an independent test facility.

The Steri-Run cycle, which includes a 2-hour heating phase, a 1.5-hour sterilization phase at 180 °C, and a cool-down phase of approximately 8 hours, has proven to be a formidable force against microorganisms. The cycle is proven to provide a total 12-log Sterility Assurance Level (SAL), meeting the stringent standards set by both the European Union and the United States.

Our application note, “Thermo Scientific Steri-Run Sterilization Cycle Proves Total Sterilization,” dives deep into the test methods and results, including how the Steri-Run cycle eliminated tough-to-eradicate microorganisms like Bacillus atrophaeus, Aspergillus niger and Mycoplasma. We also show the results of detailed temperature mapping, which is crucial to ensure all areas of the incubator consistently reach and maintain the required temperature – a key factor in sterilization.

Reading this application note will equip you with a wealth of information, including a thorough understanding of the Steri-Run cycle’s functionality and effectiveness from the scientific aspects to the practical applications, and the assurance of repeatable cell culture results.

To learn more about this technology, download and read the full application note: Thermo Scientific Steri-Run Sterilization Cycle Proves Total Sterilization. If you are already confident that you want your next CO₂ incubator to include the Steri-Run Sterilization Cycle, explore models of CO₂ incubators and their options.