Technical Resources

21980 - IMDM

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.030.00.4
L-Alanine89.025.00.28089887
L-Arginine hydrochloride211.084.00.39810428
L-Asparagine-H2O150.028.40.18933333
L-Aspartic acid133.030.00.22556391
L-Cystine240.070.00.29166666
L-Glutamic Acid147.075.00.5102041
L-Glutamine146.0584.04.0
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.0105.00.8015267
L-Leucine131.0105.00.8015267
L-Lysine hydrochloride183.0146.00.7978142
L-Methionine149.030.00.20134228
L-Phenylalanine165.066.00.4
L-Proline115.040.00.3478261
L-Serine105.042.00.4
L-Threonine119.095.00.79831934
L-Tryptophan204.016.00.078431375
L-Tyrosine disodium salt225.0104.00.46222222
L-Valine117.094.00.8034188
Vitamins
Biotin244.00.0135.327869E-5
Choline chloride140.04.00.028571429
D-Calcium pantothenate477.04.00.008385744
Folic Acid441.04.00.009070295
Niacinamide122.04.00.032786883
Pyridoxal hydrochloride204.04.00.019607844
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.04.00.011869436
Vitamin B121355.00.0139.594096E-6
i-Inositol180.07.20.04
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)147.0219.01.4897959
Magnesium Sulfate (MgSO4-2H2O176.0200.01.1363636
Potassium Chloride (KCl)75.0330.04.4
Potassium Nitrate (KNO3)101.00.0767.524752E-4
Sodium Bicarbonate (NaHCO3)84.03024.036.0
Sodium Chloride (NaCl)58.04505.077.67242
Sodium Phosphate monobasic (NaH2PO4-2H2O)156.0141.00.90384614
Sodium Selenite (Na2SeO3-5H20)263.00.01736.5779466E-5
Other Components
D-Glucose (Dextrose)180.04500.025.0
HEPES238.05958.025.033613
Phenol Red376.415.00.039851222
Sodium Pyruvate110.0110.01.0

Reference:

1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396. 

2. Iscove, N.N. and Melchers, F. (1978) J. Exper. Med., 147:923.

3. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) Virology 12:185.

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12440 - IMDM
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