Comparison of commercially-available phosphatase inhibitors

Kidney extract (25 µL; 0.5 µg/µL) was incubated with a fluorogenic substrate that measures phosphatase activity upon de-phosphorylation. Reactions were incubated for 1 hour at 37ºC and fluorescence was measured. The percent phosphatase inhibition is shown for each phosphatase inhibitor formulation.

Kidney extract (25 µL; 0.5 µg/µL) was incubated with a fluorogenic substrate that measures phosphatase activity upon de-phosphorylation. Reactions were incubated for 1 hour at 37ºC and fluorescence was measured. The percent phosphatase inhibition is shown for each phosphatase inhibitor formulation.

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