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Trans-Golgi Network 46 Antibody (PA1-1069) in IF
Immunofluorescence analysis of Trans-Golgi Network 46 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Trans-Golgi Network 46 Rabbit Polyclonal Antibody (Product ## PA1-1069) at 2µg/mlin 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ## A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product ## S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product ## R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
