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Available GeneArt® CRISPR-Cas9 genome-editing tools
The CRISPR-Cas9 system is composed of a short noncoding guide RNA (gRNA) that has two molecular components, a target-specific CRISPR RNA (crRNA) and an auxiliary trans-activating crRNA (tracrRNA). The gRNA unit guides the Cas9 protein to a specific genomic locus via base pairing between the crRNA sequence and the target sequence. Upon binding to the target sequence, the Cas9 protein induces a double-stranded break at the specific target sequence. Following CRISPR-Cas9–induced DNA cleavage, the break can be repaired by the cellular repair machinery using either nonhomologous end joining (NHEJ) or a homology-directed repair mechanism. With target specificity defined by a very short RNA-coding region, the CRISPR-Cas9 system greatly simplifies genome editing and has great promise in broad applications such as stem cell engineering, gene therapy, tissue and animal disease models, and engineering disease-resistant transgenic plants. We offer multiple solutions for genome editing, including CRISPR-Cas9 all-in-one expression vectors, Cas9 mRNA with options for producing target-specific gRNA, transfection reagents, and analysis tools.
