Cryo-EM University: Detectors and Cameras

Detectors and cameras

45:06

Detectors and Noise

Review of types of electron microscope cameras and overview of noise.
1:27:35

DQE and Nyquist

Overview and calculation of the sensitivity of a detector or detector quantum efficiency and Nyquist.
53:31

Dose Rate and Setting up and Experiment

Theoretical review of magnification and its effects on dose rate.
1:23:22

Movies and How to choose a Camera

Review of movies frames and movie modes in detectors, detector noise and overview of different cameras and setups.
56:31

Optics Summary linked to the Camera Usage

Review of the entire optics going from top to bottom from the electron gun all the way down to the detector.
1:59:41

Guidelines and Practical Examples

Practical examples of determining data collection requirements including dose rate, pixel size, pixel area, number of particles needed, images and time to data.

Falcon direct electron detector

21:24

Camera Introduction

Demonstration and introduction to scintillator-based camera settings in the TEM User Interface.
12:00

Search Mode and Binning

Demonstration of search mode and binning in a scintillator based camera in the TEM User Interface.
28:06

Image Acquisition and Dose Rate

Demonstration of image acquisition and dose rate for scintillator based camera in the TEM User Interface.

Scintillator based camera operation

19:55

Falcon Operation in Integrating Mode

Demonstration and introduction to operation of the direct electron detector, Falcon in Integrating mode in the TEM User Interface.
18:10

Falcon Operation in Counting Mode

Demonstration of the Falcon direct electron detector in counting mode in the TEM User Interface.
30:40

Falcon Dose Protector Calibration

Step by step demonstration of the Falcon direct electron detector calibration in the TEM User Interface.
3:04

Gain Reference Falcon in Integrating Mode

Demonstration of the acquiring the gain reference on the Falcon Camera in Integrating Mode.
12:50

Gain Reference Falcon in Counting Mode

Step by step demonstration of recording the gain reference on the Falcon camera in counting mode.

Energy filter and K2 DED

26:33

Energy Filtered Contrast

Review of the function of the energy filter in an electron microscope.
8:54

Energy Filtered TEM (EFTEM)

Summary of energy filtered TEM mode (EFTEM) in the TEM User Interface.
22:00

Digital Micrograph (DM)

Demonstration and introduction to Digital Micrograph.
13:53

How to use a K2 in Live Mode

Demonstration of how to use a K2 in live mode.
12:37

How to record an Image on the K2

Step by step walk through of how to record a single image on the K2.
13:41

Filter Tuning

Demonstration on how to tune the GIF (Gatan Imaging Filter) in counting mode and linear mode.
0:00

Zero Loss Imaging

Demonstration of the effect of the energy filter slit width on the image.

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection