Key learning objectives of this seminar:

  • Methods for sample preparation and LC and MS methods for high-throughput shotgun proteomics
  • MS acquisition methodology used for very deep single-shot proteomics
  • Multiplexed tandem mass tag quantification methods on the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer for TMT 10-plex

With advances in mass resolution, mass accuracy, fragmentation technology, and speed, bottom-up analysis can identify more proteins in more-complex samples than ever. Moreover, the techniques and principles involved are generally foundational for more complex experiments. Here, we evaluate the performance of the newly introduced Q Exactive HF-X MS. Various MS methods and LC gradient lengths were evaluated for bottom-up proteomics. The largest gains were found at ultrashort gradients with a fast acquisition method resulting in more than 1,100 unique peptide sequences per minute. Additional data will be shown for phosphoproteomics with tandem isobaric mass tag (TMT10-plex) and data-independent acquisition (DIA) analysis on the new Q Exactive HF-X mass spectrometer.

Sign up to watch the webinar and download the executive summary detailing:

  • The hardware and software improvements that enable the Q Exactive HF-X MS to push the speed and sensitivity limits of protein identification
gen-exec-summary-screenshot-02-450x450px

About the presenter

Jesper Olsen, Ph.D, Full Professor, Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen

Jesper Olsen, Ph.D, Full Professor
Novo Nordisk Foundation Center for Protein Research (CPR)
University of Copenhagen

Jesper Olsen studied analytical chemistry at the University of Southern Denmark in Odense, and obtained his PhD in biochemistry and molecular biology in the laboratory of Matthias Mann. During his PhD he was involved in developing high-resolution mass spectrometry-based proteomics. He spent 4 years as a post-doctoral fellow at the Max Planck Institute for Biochemistry in Munich, where he developed a quantitative phosphoproteomics technology that was applied to global time-resolved analyses of cell signaling pathways in human cells.

In 2009, Jesper was recruited back to Denmark to head a group at the newly established Novo Nordisk Foundation Center for Protein Research (CPR) at University of Copenhagen. In 2012, he was promoted to vice director of CPR and in 2014 full professor. Jesper has received a number of research awards including the Max Planck Institute for Biochemistry Junior Research Award and HUPO Young Investigator Award in Proteomic Sciences.

Overview

Key learning objectives of this seminar:

  • Methods for sample preparation and LC and MS methods for high-throughput shotgun proteomics
  • MS acquisition methodology used for very deep single-shot proteomics
  • Multiplexed tandem mass tag quantification methods on the Thermo Scientific Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer for TMT 10-plex

With advances in mass resolution, mass accuracy, fragmentation technology, and speed, bottom-up analysis can identify more proteins in more-complex samples than ever. Moreover, the techniques and principles involved are generally foundational for more complex experiments. Here, we evaluate the performance of the newly introduced Q Exactive HF-X MS. Various MS methods and LC gradient lengths were evaluated for bottom-up proteomics. The largest gains were found at ultrashort gradients with a fast acquisition method resulting in more than 1,100 unique peptide sequences per minute. Additional data will be shown for phosphoproteomics with tandem isobaric mass tag (TMT10-plex) and data-independent acquisition (DIA) analysis on the new Q Exactive HF-X mass spectrometer.

Sign up to watch the webinar and download the executive summary detailing:

  • The hardware and software improvements that enable the Q Exactive HF-X MS to push the speed and sensitivity limits of protein identification
gen-exec-summary-screenshot-02-450x450px
Presenter

About the presenter

Jesper Olsen, Ph.D, Full Professor, Novo Nordisk Foundation Center for Protein Research (CPR), University of Copenhagen

Jesper Olsen, Ph.D, Full Professor
Novo Nordisk Foundation Center for Protein Research (CPR)
University of Copenhagen

Jesper Olsen studied analytical chemistry at the University of Southern Denmark in Odense, and obtained his PhD in biochemistry and molecular biology in the laboratory of Matthias Mann. During his PhD he was involved in developing high-resolution mass spectrometry-based proteomics. He spent 4 years as a post-doctoral fellow at the Max Planck Institute for Biochemistry in Munich, where he developed a quantitative phosphoproteomics technology that was applied to global time-resolved analyses of cell signaling pathways in human cells.

In 2009, Jesper was recruited back to Denmark to head a group at the newly established Novo Nordisk Foundation Center for Protein Research (CPR) at University of Copenhagen. In 2012, he was promoted to vice director of CPR and in 2014 full professor. Jesper has received a number of research awards including the Max Planck Institute for Biochemistry Junior Research Award and HUPO Young Investigator Award in Proteomic Sciences.

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