Video Tutorials for Immunology Research

Translational research focused on drug-resistant and emerging pathogens requires robust and reliable cell-based assays and validated analysis systems. In this seminar, a variety of techniques for bacterial and viral analysis will be demonstrated in cell-based models using powerful cytometric instrumentation. Special focus will be given to assays to characterize bacteria and the immune response to bacteria. Additionally, discussion will include methods to evaluate viral infection and proliferation in host cells. Recent innovations in high-throughput flow cytometry, partnered with regulatory-compliant acquisition and analysis software, will provide valuable additions to your portfolio of assays for microbial research.

Let’s get ready to immunophenotype! Panel building usually requires many steps for optimal experimental design. In this session, learn the 5 steps for building a quick and easy experiment to find your immune cell subsets.

Monoclonal antibody therapeutics have proven to be a robust and effective modality to treat a range of diseases. Many therapeutic antibodies such as Rituximab have seen clinical success in treating cancer. Though some antibodies can kill cancer cells directly, the primary mechanism of action is often via antibody-dependent killing by immune cells or complement protein complexes in the blood. In vitro assays to characterize antibody-dependent cell killing commonly rely on preparations of isolated immune cells such as PBMCs or isolated T- or NK-cells. While these models provide a reliable cell-based system to characterize antibody function, they may be biased by the absence of components present in whole blood. We describe new flow cytometry methods to characterize immune cell killing and use these assays to evaluate antibody-dependent killing of leukemia cells with isolated T and NK cells compared to whole blood.