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            • IFN gamma ELISA Kits
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            Invitrogen

            Human IFN gamma ELISA Development Kit (ABTS), PeproTech®

            View all (37) IFN gamma ELISA Kits
            Technical guide & protocol Tech Support
            Catalog # 900-K27K
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            Price (EUR)
            393,00
            10 x 96 Tests
             
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              Human IFN gamma ELISA Development Kit (ABTS)
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              Human IFN gamma ELISA Development Kit (ABTS)

              Human IFN gamma ABTS ELISA standard curve (Product # 900-K27K).
              View Product

              Human IFN gamma ELISA Development Kit (ABTS)
              Human IFN gamma ELISA Development Kit (ABTS) (900-K27K)
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              Product Specifications

              Analytical sensitivity

              Lot-specific

              Assay range

              Lot-specific

              Hands-on time

              1 hr 45 min

              Time-to-result

              24 hr 30 min

              Homogenous (no wash)

              No

              Instrument

              Absorbance Microplate Reader

              Product size

              10 x 96 Tests

              Contents

              This Human IFN gamma ELISA Kit includes Capture Antibody, Detection Antibody, Protein Standard, and HRP-conjugate. The HRP-conjugate is packaged separately.

              Storage

              Refer to product documentation for component specific storage temperature.

              Protein name

              IFN gamma

              Protein family

              Growth Factors & Receptors

              Species (tested)

              Human

              Assay kit format

              ELISA Development Kit

              Detector antibody conjugate

              Biotin

              Label or dye

              HRP

              About This Kit

              900-K27K consists of: 900-K27 (capture antibody, detection antibody, and protein standard), and AVIDIN-60UL (avidin HRP-conjugate).

              Human IFN gamma ABTS ELISA Development Kit contains the key components required for the quantitative measurement of natural and/or recombinant human IFN gamma in a sandwich ELISA format. Human IFN gamma ABTS ELISA Development Kit contains enough material to assay the target cytokine in at least 1,000 ELISA plate wells.

              The ABTS ELISA Buffer Kit (Product # 900-K00), sold separately, is recommended to be used in conjunction with this ABTS ELISA Development Kit for optimal performance. This buffer kit contains all the necessary components to assay ten 96-well ELISA plates (included) and contains detailed handling instructions.

              Target information

              IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

              For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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              Cite this product

              Bioinformatics

              Gene aliases : IFG, IFI, IFNG

              Gene ID : (Human) 3458

              Gene symbol : IFNG

              Protein Aliases : IFN-gamma, IFNγ, Interferon, Interferon y, Interferon γ, Interferonγ, M-IFN-g, R-IFN-g, H-IFN-g, IFN γ, IFN-y, Immune interferon, Interferon gamma, interferon, gamma

              UniProt ID (Human) P01579

              Recommended Products

              IL-6 Human Matched Antibody Pair

              Because you are interested in IL-6 Human ELISA Kit.

              SIZE

              10 x 96 tests

              PRICE

              USD 400.00

              Cat # CHC1263

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