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Invitrogen
Basic Human PD-1 ELISA Kit
Product Specifications
Analytical sensitivity
1.14 pg/mL
Assay range
2.34-150 pg/mL
Sample type/volume
Supernatant
50 µL
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PD-1 (CD279)
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Kit Overview
The Human PD-1 ELISA kit enables the quantification of Human PD-1 in various biological samples, including human serum, plasma, buffered solutions, and cell culture medium. This assay is specifically designed to exclusively detect both natural and recombinant forms of Human PD-1.
Principle of the assay
The Human PD-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) follows a unique approach to measure the amount of the target molecule. It utilizes a matched pair of antibodies to capture the target and form a sandwich complex. The first antibody is pre-coated on the microplate wells, where samples, standards, or controls are added and bind to the immobilized antibody. The second antibody, acting as the detector, is then introduced, followed by the addition of a substrate solution that generates a measurable signal upon interaction with the enzyme-antibody-target complex. The intensity of this signal directly correlates to the concentration of the target present in the original specimen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD279, hPD-1, hPD-l, hSLE1, PD-1, PD1, PDCD1, SLEB2
Gene ID : (Human) 5133
Gene symbol : PDCD1
Protein Aliases : CD279, hPD1, Protein PD1, sCD279, soluble CD279, programmed cell death 1 protein, Programmed cell death protein 1, Protein PD-1, systemic lupus erythematosus susceptibility 2
UniProt ID (Human) Q15116
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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