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Product Specifications
Analytical sensitivity
1.3 pg/mL
Assay range
7.8-500 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Plasma
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-4
Protein family
Hormones & Receptors
Species (tested)
Human
Species (reported)
Human
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Interleukin-4 (Hu IL-4) ELISA kit is a powerful tool for accurately quantifying Hu IL-4 in a variety of biological samples, including human serum, plasma, urine, or cell culture medium. This highly specific assay is designed to exclusively recognize and measure both endogenous and recombinant forms of Hu IL-4.
Principle of the method
The Human IL-4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) employs a meticulously selected pair of antibodies to capture and measure the target analyte. Within the microplate wells, a specific antibody that binds specifically to the target is pre-coated. Upon the addition of samples, standards, or controls, they bind to the immobilized (capture) antibody within the wells. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex, resulting in the generation of a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
Target information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4
Gene ID : (Human) 3565
Gene symbol : IL4
Protein Aliases : B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, H-IL-4, ILN, Interleukin, Interleukin4, MGC79402, M-IL-4, IL-4, interleukin 4 variant 2, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra
UniProt ID (Human) P05112
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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