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Human Gastrin ELISA Kit
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Product Specifications
Analytical sensitivity
9.38 pg/mL
Assay range
15.63-1,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Gastrin
Protein family
Gastrin/cholecystokinin
Species (tested)
Human
Assay kit format
Competitive ELISA
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Gastrin (GT) ELISA quantitates GT in serum, plasma and other biological fluids
Principle of the method:
The Competitive Gastrin (GT) ELISA research-use-only kit is designed to quantitatively measure GT in Humans. A GT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human GT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
This antibody may be used to study the differential tissue expression and intracellular and subcellular localization of gastrin in neuroendocrine cells of the gastrointestinal tract, and in the CNS. Antibodies to gastrin are also useful for the identification and detection of gastrin in normal and neoplastic tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : GAS, GAST
Gene ID : (Human) 2520
Gene symbol : GAST
Protein Aliases : Gastrin, gastrin-1, preprogastrin
UniProt ID (Human) P01350
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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