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Product Specifications
Analytical sensitivity
<0.43 pg/mL
Assay range
0.7-480 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr
Time-to-result
2 hr 30 min
Homogenous (no wash)
No
Interassay CV
6.8%
Intraassay CV
6.2%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Detection antibody (monoclonal antibody to human Granzyme B)
Biotin-Conjugate anti-human Granzyme B monoclonal antibody
Streptavidin-HRP
human Granzyme B Standard lyophilized
Wash Buffer Concentrate 20x
Dilution Buffer Concentrate 5x
Substrate Solution
Stop Solution
Adhesive Films
Standard
Detection antibody (monoclonal antibody to human Granzyme B)
Biotin-Conjugate anti-human Granzyme B monoclonal antibody
Streptavidin-HRP
human Granzyme B Standard lyophilized
Wash Buffer Concentrate 20x
Dilution Buffer Concentrate 5x
Substrate Solution
Stop Solution
Adhesive Films
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Granzyme B
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Granzyme B ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of human Granzyme B.
Principle of the method
The Human Granzyme B ELISA Kit (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, GRB, GZMB, HLP, SECT
Gene ID : (Human) 3002
Gene symbol : GZMB
Protein Aliases : C11, Cathepsin G-like 1, CTLA-1, CTSGL1, cytotoxic serine protease B, Cytotoxic T-lymphocyte proteinase 2, cytotoxic T-lymphocyte-associated serine esterase 1, fragmentin 2, Fragmentin-2, granzyme 2, Granzyme B, granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), Granzyme-2, HLP, Human lymphocyte protein, Lymphocyte protease, SECT, T-cell serine protease 1-3E
UniProt ID (Human) P10144
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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