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Invitrogen
Human IL-7 R alpha/CD127 ELISA Kit
Product Specifications
Analytical sensitivity
0.14 ng/mL
Assay range
0.14-100 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CD127
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human IL-7 R alpha/CD127 quantitates human IL-7 R alpha/CD127 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human IL-7 R alpha/CD127.
Target information
CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : CD127, CDW127, IL-7R-alpha, IL7R, IL7RA, ILRA
Gene ID : (Human) 3575
Gene symbol : IL7R
Protein Aliases : CD127, CD127 antigen, CDw127, IL 7 receptor, IL 7R a, IL 7R subunit alpha, IL 7R α, IL 7Ra, IL 7Rα, soluble IL 7R, IL7R α, IL7Rα, Interleukin7 receptor subunit alpha, MGC107557, sCD127, soluble IL 7 receptor, IL7 receptor, IL7 receptor subunit alpha, IL-7R, IL7R a, IL7R alpha, IL7R subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, Interleukin-7 receptor subunit alpha
UniProt ID (Human) P16871
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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