Technical guide & protocol Tech Support

Product Specifications

Analytical sensitivity

0.1 ng/mL

Assay range

0.1-25 ng/mL

Sample type/volume

Supernatant
50 µL
Serum
50 µL
Plasma
50 µL

Hands-on time

1 hr 20 min

Time-to-result

4 hr 45 min

Homogenous (no wash)

No

Interassay CV

<12%

Intraassay CV

<10%

Instrument

Colorimetric Microplate Reader

Product size

96 Tests

Contents

Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers

Shipping conditions

Wet ice

Storage

2-8°C

Protein name

ISL1

Species (tested)

Human

Assay kit format

Sandwich ELISA Kit

Detector antibody conjugate

Biotin

Label or dye

HRP

About This Kit

Human Islet-1 quantitates human Islet-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Islet-1.

Target information

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Gene aliases : Isl-1, ISL1, ISLET1

Gene ID : (Human) 3670

Gene symbol : ISL1

Protein Aliases : Insulin gene enhancer protein ISL-1, ISL1 transcription factor, LIM/homeodomain, Islet-1

UniProt ID (Human) P61371

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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