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Product Specifications
Analytical sensitivity
<2 pg/mL
Assay range
15.6-1,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr
Homogenous (no wash)
No
Interassay CV
7.1%
Intraassay CV
6.5%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Standard Dilution Buffer
Incubation Buffer
Biotinylated Detection Antibody
Strepavidin-HRP conjugate
HRP diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive plate covers
Standard
Standard Dilution Buffer
Incubation Buffer
Biotinylated Detection Antibody
Strepavidin-HRP conjugate
HRP diluent
Wash Buffer
Chromogen
Stop Solution
Controls
Adhesive plate covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
CCL3 (MIP-1 alpha)
Protein family
Chemokines & Receptors
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
The Human Macrophage Inflammatory Protein-1 alpha/Chemokine Ligand 3 (Hu MIP-1α/CCL3) ELISA quantitates Hu MIP-1α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MIP-1α.
Principle of the method
The Human MIP-1αsolid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Target information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Gene aliases : CCL3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
Gene ID : (Human) 6348
Gene symbol : CCL3
Protein Aliases : C-C motif chemokine 3, chemokine (C-C motif) ligand 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, mip1 alpha, RP23-320E6.7, H-MIP-1-alpha, LD78-alpha, PAT 464.1, SIS-beta, small inducible cytokine A3 (homologous to mouse Mip-1a), Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
UniProt ID (Human) P10147
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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