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Invitrogen
Human Pancreatic Polypeptide/PP ELISA Kit
Product Specifications
Analytical sensitivity
4 pg/mL
Assay range
4-1,000 pg/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
PPY
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Pancreatic Polypeptide/PP quantitates human Pancreatic Polypeptide/PP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Pancreatic Polypeptide/PP.
Target information
The PPY gene encodes pancreatic polypeptide, a peptide hormone involved in the regulation of appetite and energy balance. The origin of the PPY gene is linked to a tandem duplication of the PYY gene, occurring concomitantly with the emergence of tetrapods, leading to the distinct PPY function within the neuropeptide Y family. In primates and ungulates, a gene duplication event resulted in the formation of a PYY2-PPY2 gene cluster, highlighting the evolutionary complexity of these genes. The PPY gene product is secreted by gamma-cells in the pancreas, localized primarily in the islet periphery, and plays a crucial role in metabolic homeostasis by interacting with receptors in the central nervous system to modulate satiety signals postprandially. PPY's function is linked to its inhibition of AGRP/NPY neurons which decreases their suppressive effect on POMC neurons, enhancing POMC activity and contributing to appetite suppression. This intricate regulation underscores the importance of PPY in dietary intake and metabolic disorders, with ongoing research exploring its potential therapeutic applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : PNP, PP, PPY
Gene ID : (Human) 5539
Gene symbol : PPY
Protein Aliases : HGNC:9327, IOPPP, PP1, PPA1, PPase, SID6-8061, Pancreatic polypeptide prohormone, Pancreatic polypeptide Y, PH, prepro-PP (prepropancreatic polypeptide)
UniProt ID (Human) P01298
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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