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Invitrogen
Human Pro-Cathepsin B ELISA Kit
Product Specifications
Analytical sensitivity
61 pg/mL
Assay range
61-15 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
Pro-Cathepsin B
Species (tested)
Human
Assay kit format
Sandwich ELISA Kit
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Pro-Cathepsin B quantitates human Pro-Cathepsin B in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Pro-Cathepsin B.
Target information
Pro-Cathepsin B is the inactive precursor of Cathepsin B, a lysosomal cysteine protease that plays a significant role in both physiological and pathological processes. Synthesized in the endoplasmic reticulum, pro-Cathepsin B undergoes maturation in the acidic environment of the lysosome, where it is activated. Structurally, Cathepsin B consists of a heavy chain and a light chain forming a bi-lobal enzyme with a critical region known as the "occluding loop," which dictates its substrate specificity and activity. Functionally, Cathepsin B is involved in intracellular protein turnover, aiding in the degradation of proteins within endosomes and lysosomes. It also has crucial roles in extracellular matrix remodeling, facilitating processes such as cell migration, invasion, and angiogenesis, particularly in the context of cancer. Dysregulation of Cathepsin B activity is linked to various diseases, including tumor metastasis, neurodegenerative disorders, and inflammatory conditions, making it an important target for therapeutic intervention.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : APPS, CPSB, CTSB
Gene ID : (Human) 1508
Gene symbol : CTSB
Protein Aliases : amyloid precursor protein secretase, APP secretase, APPS, Cathepsin B, Cathepsin B1, cysteine protease, ProCTSB
UniProt ID (Human) Q96D87
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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