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Product Specifications
Analytical sensitivity
1.2 pg/mL
Assay range
7.8-500 pg/mL
Sample type/volume
Serum
50 µL
Supernatant
50 µL
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Shipping conditions
Wet ice
Storage
2-8°C
Protein name
IL-1 beta
Protein family
Hormones & Receptors
Species (tested)
Mouse
Species (reported)
Mouse
Assay kit format
Basic ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The mouse Interleukin-1beta (IL-1beta) ELISA kit enables the quantitative measurement of mouse IL-1beta in a variety of biological samples, including murine serum, plasma, urine, and cell culture medium. This assay is specifically designed to accurately detect both natural and recombinant forms of mouse IL-1beta.
Principle of the method
The mouse IL-1beta solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) utilizes a well-matched pair of antibodies to capture and quantify the target molecule. The microplate wells are pre-coated with a specific antibody that binds to the target. Samples, standards, or controls are then added to the wells, allowing them to bind to the immobilized (capture) antibody. A second (detector) antibody is subsequently added, forming a sandwich complex. The addition of a substrate solution triggers a reaction with the enzyme-antibody-target complex, resulting in the production of a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
Target information
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : Il-1b, IL-1beta, Il1b
Gene ID : (Mouse) 16176
Gene symbol : Il1b
Protein Aliases : IL-1 beta, Catabolin, Hematopoietin 1 (H1), IFN beta inducing factor, il 1b, IL 1β, IL1 B, IL1β, Osteoclast activating factor (OAF), Pro interleukin 1 beta, ILN, Interleukin, Interleukin 1 beta precursor, Interleukin1 beta, Interleukin-1b, LAF, Interleukin-1 beta
UniProt ID (Mouse) P10749
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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